Drought-Mediated Modulation in Metabolomic Profiling of Nigella Sativa Leaf, Growth, Ecophysiology and Antioxidants  

作　　者：Khalid Rehman Hakeem Hesham F.Alharby M.Irfan Qureshi 

机构地区：[1]Department of Biological Sciences,Faculty of Science,King Abdulaziz University,Jeddah,Saudi Arabia [2]Princess Dr.Najla Bint Saud Al-Saud Center for Excellence Research in Biotechnology,King Abdulaziz University,Jeddah,Saudi Arabia [3]Department of Public Health,Daffodil International University,Dhaka,Bangladesh [4]Department of Biotechnology,Jamia Millia Islamia,New Delhi,India

出　　处：《Phyton-International Journal of Experimental Botany》2023年第12期3323-3344,共22页国际实验植物学杂志（英文）

基　　金：funded by the Deanship of Scientific Research (DSR)at King Abdulaziz University,Jeddah,under Grant No.G:243-130-1439.

摘　　要：Abiotic stresses,including drought,have been found to affect the growth and medicinal quality of numerous herbs.The proposed study aims to study the effects of different drought regimes on the metabolic profile,growth,ecophysiology,cellular antioxidants,and antioxidant potential of Nigella sativa(Black cumin)leaf.Forty-day-old seedlings of N.sativa were exposed to three regimes of drought(control,moderate and high)for a week.UPLCMS/MS metabolic profile of the leaf reveals the presence of more than a hundred metabolites belonging to anthocyanins,chalcones,dihydro flavonoids,flavonoids,flavanols,flavones,flavonoid carbonoside,isoflavones,etc.Drought was found to alter the contents of identified metabolites.Drought stress-induced oxidative stress and increased production of hydrogen peroxide and superoxide anions.Physiological changes,activities of antioxidant enzymes,contents of antioxidants,and proline were significantly high under drought to protect against the low water regimes.Furthermore,stressed leaf extract had higher antioxidant potential.Thus,N.sativa leaf bears multiple metabolic pathways and can tolerate a higher degree of drought or osmotic stress.

关 键 词：Nigella sativa metabolomics DROUGHT antioxidant mechanism FLAVONOIDS 

分 类 号：Q94[生物学—植物学]