双水相法萃取灵芝醇提液中三萜类化合物的工艺优化  

The Optimized Process for the Extraction of Triterpenoids from the Alcoholic Extract of Ganoderma lucidum with Aqueous Two-Phase System

作　　者：韩伟 姚泽远 卜原玲 王承潇[3] HAN Wei;YAO Zeyuan;BU Yuanling;WANG Chengxiao(Engineering Research Center of Pharmaceutical Process Chemistry,Ministry of Education,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;School of Life Science,Kunming University of Science and Technology,Kunming 650000,China)

机构地区：[1]华东理工大学药学院制药工程与过程化学教育部工程研究中心,上海200237 [2]华东理工大学药学院上海市新药设计重点实验室,上海200237 [3]昆明理工大学生命科学与技术学院,云南昆明650000

出　　处：《徐州工程学院学报（自然科学版）》2023年第4期5-13,共9页Journal of Xuzhou Institute of Technology(Natural Sciences Edition)

基　　金：国家自然科学基金项目(82060645);上海市科技兴农重点攻关资助项目(沪农科创字(2018)第1-1号)。

摘　　要：通过构建无机盐-乙醇双水相体系萃取灵芝三萜类化合物.考察了13种无机盐与乙醇构成的醇-盐体系对灵芝三萜萃取效率,发现NaH_(2)PO_(4)-乙醇体系萃取率最高.采用单因素试验考察了NaH_(2)PO_(4)水溶液质量浓度、乙醇和NaCl质量分数、水相pH对回收率的影响,并通过正交试验得到优化的工艺条件:NaH_(2)PO_(4)、水、乙醇、灵芝醇提液的质量分数分别为10%、53%、27%和10%,水相pH值为5.同时还比较了双水相和碱提酸溶2种分离纯化方法,发现双水相法灵芝三萜萃取率为47.366%,高于碱提酸溶法的44.933%,且双水相法萃取过程更为简便绿色.The method of extracting triterpenoids from alcohol extract of Ganoderma lucidum was studied by constructing the aqueous two-phase system of salt and alcohol.The extraction efficiency of Ganoderma lucidum triterpenes was investigated by an alcohol-salt system consisting of 13 inorganic salts and ethanol.It was found that the EtOH-NaH_(2)PO_(4) system had the highest extraction efficiency.The effects of NaH_(2)PO_(4) concentration in aqueous solution,mass fractions of ethanol and NaCl,and pH of aqueous phase on extraction efficiency were investigated by single factor experiment.The optimized process conditions were obtained by orthogonal experiment:the mass fractions of NaH_(2)PO_(4),water,ethanol and Ganoderma lucidum alcohol extract were 10%,53%,27%and 10%,respectively.Before extraction,solid NaOH was used to adjust the pH of aqueous phase to 5.At the same time,the results of the two methods of extraction,namely,the aqueous two-phase and the alkaline-acid solvent,were also compared.It was found that the extraction rate of triterpenes of Ganoderma lucidum by the aqueous two-phase method was 47.366%,which was higher than that by the alkaline extraction and acid solution(44.933%).Furthermore,the aqueous two-phase extraction was proved to be an efficient and green extraction method.

关 键 词：灵芝三萜 双水相体系 萃取 正交试验 工艺优化 

分 类 号：Q946-3[生物学—植物学]