不同果色辣椒CCS基因克隆及表达分析  

Cloning and Expression Analysis of CCS Gene in Pepper with Different Fruit Color

作　　者：李全辉[1] 马玉杰 邵登魁[1] 王亚艺[1] 文军琴 钟启文[1] LI Quanhui;MA Yujie;SHAO Dengkui;WANG Yayi;WEN Junqin;ZHONG Qiwen(Qinghai Academy of Agricultural and Forestry Sciences/Laboratory of Germplasm Resources Research and Utilization of the Qinghai-Tibet Plateau,Xining 810016,China)

机构地区：[1]青海大学农林科学院/青藏高原种质资源研究与利用实验室,西宁810016

出　　处：《西北植物学报》2023年第12期2030-2038,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：青海省科技计划项目(2021-ZJ-763)。

摘　　要：果实颜色是辣椒重要的商品性状之一。研究以观赏椒GS6、Z1,甜椒SP01及黄色突变体SP02为材料,探究辣椒红素/玉红素合酶基因(CCS)在不同成熟果色辣椒中的序列差异和表达特性,初步解析辣椒不同成熟果色形成分子机理。研究结果显示,成熟色为红色的GS6、Z1和SP01中均能克隆到CCS全长基因,且序列无差异,其全长1497 bp,编码498个氨基酸,只包含1个开放阅读框序列,没有内含子序列;而黄色突变体SP02中未能克隆出CCS基因;聚类和系统进化分析发现辣椒CCS基因与茄科作物的番茄、中华辣椒和灯笼辣椒等植物的亲缘关系较近;qRT-PCR分析结果显示:在GS6中,CCS基因在花中的表达量最高;在Z1和SP01中,CCS基因在果实中的表达量显著高于其他组织,在根中表达量最低;而在SP01的茎和叶以及SP02的所有组织中,CCS基因均未表达。在果实不同发育时期,CCS基因在SP01花后30 d(Ⅲ期)、GS6和Z1花后40 d(Ⅳ期)表达量显著上升。研究结果表明,甜椒黄色突变体SP02果实颜色的形成可能和CCS基因的缺失或变异密切相关,而在成熟色为红色的辣椒中CCS基因的表达可能在果实颜色形成过程中发挥重要作用。Fruit color is one of the important commercial traits of pepper.In this study,the authors used ornamental pepper GS6,Z1,sweet pepper SP01 and its yellow mutant SP02 as materials to explore the sequence differences and expression characteristics of capsanthin/capsorubin synthase(CCS)gene in pepper with different mature fruit colors,and to preliminarily analyze the molecular mechanism of pepper with different mature fruit colors.The results showed that the full-length CCS gene could be cloned from GS6,Z1 and SP01 with red mature color,and there was no difference in the sequence.The full-length of CCS gene was 1497 bp,encoding 498 amino acids,containing only one open reading frame sequence and no intron sequence.The CCS gene was not cloned in the yellow mutant SP02.Clustering and phylogenetic analysis showed that the CCS genes of Capsicum annuum were more closely related to C.chinense,C.baccatum,and Solanum pennellii of Solanaceae crops.The results of qRT-PCR analysis showed that the expression of CCS gene was the highest in flowers in GS6.In Z1 and SP01,the expression level of CCS gene in fruit was significantly higher than that in other tissues, and the expression level in root was the lowest. The CCS gene was not expressed in the stems and leaves of SP01 and all tissues of SP02. At dif-ferent fruit development stages, the expression of CCS gene increased significantly at 30 days after flower-ing (stage Ⅲ) of SP01, 40 days after flowering (stage Ⅳ) of GS6 and Z1. The results showed that the for-mation of fruit color of sweet pepper yellow mutant SP02 might be closely related to the deletion or varia-tion of CCS gene, and the expression of CCS gene in mature red pepper might play an important role in fruit color formation.

关 键 词：辣椒 辣椒红素/玉红素合成酶 基因克隆 类胡萝卜素 

分 类 号：Q786[生物学—分子生物学] Q946[农业科学—蔬菜学] S641.3[农业科学—园艺学]