精液检验室间质量评价统计学方案的比对研究  

A comparative study of statistical protocols for external quality assessment of semen

作　　者：伍细言 彭新华 李维娜 贺景亮 吴慧兰 朱文兵 陆金春[6] WU Xiyan;PENG Xinhua;LI Weina;HE Jingliang;WU Huilan;ZHU Wenbing;LU Jinchun(Center for Reproductive Medicine,Hunan Provincial Maternal and Child Health Care Hospital,Changsha 410008,Hunan;Department of Andrology,Reproductive and Genetics Hospital of CITIC-Xiangya,Changsha 410221,Hunan;Human Sperm Bank,Reproductive and Genetics Hospital of CITIC-Xiangya,Changsha 410221,Hunan;Center for Reproductive Medicine,Xiangya Hospital of Central South University,Changsha 410008,Hunan;Institute of Reproductive and Stem Cell Engineering,Central South University,Changsha 410078,Hunan;Center for Reproductive Medicine,Zhongda Hospital,Southeast University,Nanjing 210037,Jiangsu,China)

机构地区：[1]湖南省妇幼保健院生殖医学中心,长沙410008 [2]中信湘雅生殖与遗传专科医院男科学部,长沙410221 [3]中信湘雅生殖与遗传专科医院人类精子库,长沙410221 [4]中南大学湘雅医院生殖医学中心,长沙410008 [5]中南大学生殖与干细胞工程研究所,长沙410078 [6]东南大学附属中大医院生殖医学中心,南京210037

出　　处：《临床检验杂志》2023年第10期778-781,共4页Chinese Journal of Clinical Laboratory Science

基　　金：湖南省妇幼保健院锐新项目(2021RX30);湖南省自然科学基金面上项目(2022JJ30018)。

摘　　要：目的 通过比较不同统计学方案对精液检验室间质评结果的影响,选择合适的统计学方案进行推广。方法 以精子浓度为例,选择2022年湖南省20家实验室精液室间质评数据,分别用传统统计方案、稳健统计方案以及结合稳健统计技术剔除“离群值”后的传统统计进行分析比对,比较其优劣。结果 4组精子浓度数据经传统统计无法剔除“离群值”,与Robust稳健统计方法或结合稳健统计技术剔除“离群值”后的传统统计室间质评结果有差异,传统统计与稳健统计合格实验室数分别为:19 vs 16、19 vs 16、19 vs 19、19 vs 19。结论 传统统计方法不适用于不符合正态分布、数据量较小、可能存在较多“离群值”的精液检验室间质评统计,推荐使用Robust稳健统计方法;或结合稳健统计技术剔除“离群值”后,可得到与稳健统计方案相似的结果。Objective To compare the effects of different statistical protocols on the results of external quality assessment(EQA)of semen,and select appropriate statistical protocols for the promotion of EQA of semen.Methods Taking sperm concentration as an example,the semen EQA data of 20 laboratories in Hunan Province in 2022 were selected,and the advantages and disadvantages of the traditional statistical scheme(TSS),robust statistical scheme(RSS)and traditional statistical scheme after eliminating the"outliers"(TSEOS)combined with robust statistical technology were analyzed and compared.Results The"outliers"could not be excluded from the sperm concentration data of the four groups in the TSS,which led to the difference between TSS and RSS or TSEOS.The number of qualified laboratories for TSS and RSS were 19 vs 16,19 vs 16,19 vs 19,and 19 vs 19,respectively.Conclusion The results of RSS are similar to those of TSEOS.Compared with TSS,RSS do not need to remove outlier data steps,and are more suitable for semen EQA data analysis with small data volume.

关 键 词：精子浓度 室间质量评价 稳健统计 离群值 靶值 可接受区间 

分 类 号：R446[医药卫生—诊断学]