玫瑰石斛的化学成分及其生物活性  

Chemical Compounds Isolated from Dendrobium crepidatum and Their Bioactive Activities

作　　者：庄晨曦 王慧敏 陈亚萍 张锐[1,3] 郑金蓉 李美红[1,3] 李玉鹏 ZHUANG Chenxi;WANG Huimin;CHEN Yaping;ZHANG Rui;ZHENG Jinrong;LI Meihong;LI Yupeng(School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming Yunnan 650500;School of Pharmacy,Honghe Health Vocational College,Honghe Yunnan 661100;College of Modern Biomedical Industry,Kunming Medical University,Kunming Yunnan 650500;Dept.of Pharmacy,Yunnan Infectious Disease Hospital,Kunming Yunnan 650301,China)

机构地区：[1]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500 [2]红河卫生职业学院药学院,云南红河661100 [3]昆明医科大学现代生物医药产业学院,云南昆明650500 [4]云南传染病医院药剂科,云南昆明650301

出　　处：《昆明医科大学学报》2023年第12期1-5,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(21202066);云南省科技厅-昆明医科大学联合专项基金资助项目(202301AY070001-212);云南省本科教育教学改革研究基金资助项目(JG2023001);昆明医科大学教研教改基金资助项目(2022-JY-S-08);云南省创新团队基金资助项目(202005AE160004);云南省天然药物药理重点实验室开放研究基金资助项目(YKLPNP-G2407)。

摘　　要：目的研究玫瑰石斛化学成分,发现其抗肿瘤活性成分。方法玫瑰石斛经甲醇提取、硅胶和凝胶柱色谱分离,波谱分析确定结构。MTT法进行部分化合物体外抗肿瘤活性筛选。结果9个化合物从玫瑰石斛中分离鉴定,分别为p-hydroxybenzaldehyde(1)、p-methoxyphenol(2)、p-Hydroxybenzyl alcohol(3)、hydroquinone(4)、(24R)-ethylcholest-5-en-3-ol-7-one(5)、sinalexin(6)、bis(2-ethylhexyl)benzene-1,2-dicarboxylate(7)、pinoresinol(8)、Chaenomin B(9),对化合物5~9进行抗肿瘤活性测试,发现化合物9对A549细胞株抑制活性较好。结论化合物2~5,7~8为首次从本植物中分离得到,化合物6,9为首次从本属植物中分离得到,9对A549细胞株IC_(50)为29.35μM。Objective To study the chemical constituents from the herbs of Dendrobium crepidatum and identify its antitumor bioactive compounds.Methods The constituents were extracted by methyl alcohol and isolated by CC and Sephadex LH-20.Their structures were identified by spectroscopic analysis(1H NMR,13CNMR and EIMS).The antitumor activity of the constituents was determined by MTT assay in vitro.Results 9 compouds were obtained and identified as p-hydroxybenzaldehyde(1),p-methoxyphenol(2),p-Hydroxybenzyl alcohol(3),hydroquinone(4),(24R)-ethylcholest-5-en-3-ol-7-one(5),sinalexin(6),bis(2-ethylhexyl)benzene-1,2-dicarboxylate(7),pinoresinol(8),Chaenomin B(9).The antitumor activity of compounds 5~9 was tested,and it was found that compound 9 had good inhibitory activity against A549 cell line.Conclusion Compounds 2~5,7~8 were isolated from the herbs for the first time and compounds 6,9 were isolated from this genus for the first time.IC_(50)of 9 on A549 cell line is 29.35μM.

关 键 词：玫瑰石斛 化学成分 生物活性 

分 类 号：R284.2[医药卫生—中药学]