甘草汁蒸制高乌头后“减毒存效”部位化学成分UPLC-Q-TOF-MS分析  

UPLC-Q-TOF-MS analysis of the chemical composition of the'detoxification and efficiency enhancement'part of Aconitum sinomontanum Nakai steamingwith licorice juice

作　　者：贾春艳 强宇靖[1] 杨锡仓 苗小楼[3] 李芸[1] JIA Chunyan;QIANG Yujing;YANG Xicang;MIAO Xiaolou;LI Yun(Gansu University of Chinese Medicine,Lanzhou 730000,China;Affiliated Hospital of Gansu University of Chinese Medicine,Lanzhou 730020,China;Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China)

机构地区：[1]甘肃中医药大学,兰州730000 [2]甘肃中医药大学附属医院,兰州730020 [3]中国农业科学院兰州畜牧与兽药研究所,兰州730050

出　　处：《中华中医药杂志》2023年第12期5971-5980,共10页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81560650)。

摘　　要：目的:分析高乌头炮制前后三氯甲烷部位化学成分并揭示炮制后化学成分的转化机制。方法:通过系统溶剂法制备高乌头炮制前后三氯甲烷部分,采用UPLC-Q-TOF-MS技术对比研究高乌头炮制前后三氯甲烷部位化学成分变化规律;采用模拟炮制技术,发现高乌甲素及冉乌头碱炮制前后发生质变与量变特征成分的转化机制,从而揭示甘草汁蒸制高乌头后“减毒存效”的炮制原理。结果:高乌头炮制后化学成分组成及比例发生明显变化,其中有7个成分消失,新增30个成分。从生品三氯甲烷部分中检测到30个化合物,共鉴定出9个;从制品三氯甲烷部分中检测到53个化合物,其中有30个为新增成分,共鉴定出13个。对比炮制前后化学成分,发现炮制后消失的成分为8-O-acetylexcelsine,推测此成分可能为毒性成分;炮制后新增成分有高乌宁乙、L49/50、L6、L19、邻氨基苯甲醛,可能为药效物质基础。结论:高乌头炮制后发生质变与量变特征成分的转化途径有多种,这些转化途径可能为甘草汁蒸制高乌头的解毒途径。Objective:To analyse the chemical composition of the trichloromethane parts of Aconitum sinomontanum Nakai before and after steaming with licorice juice,and reveal the chemical composition transformation mechanism after processing.Methods:The systematic solvent method was used to prepare the chloroform parts of Aconitum sinomontanum Nakai before and after processing.UPLC-Q-TOF-MS technique was used to study the chemical composition and change rule of the chloroform parts of Aconitum sinomontanum Nakai after process.The simulated processing technology was used to explore the transformation mechanism of qualitative and quantitative changes of lappaconitine and ranaconidine before and after processing,and the processing principle of'reducing toxicity and preserving efficiency'of Aconitum sinomontanum Nakai was revealed.Results:There were significant differences in the chemical composition of chloroform part before and after processing,and the chemical composition and proportion of chloroform part changed obviously after processing,in which 7 components disappeared and 30 new components were added.Thirty compounds were detected from raw Aconitum sinomontanum Nakai chloroform part and 9 components were identified.A total of 53 compounds were detected in the chloroform part of the process product,among which 30 compounds were new added and a total of 13 components were identified.Comparing the chemical constituents before and after processing,it was found that the common constituents were Lappaconitine,Ranaconidine,N-deacetyllappaconitine,Ranaconitine,lappaconine,sinomontanine H and L18.After processing the 8-O-acetylexcelsine missed,which was speculated to be a toxic ingredient.After processing,the newly added ingredients were sinomontanine B,L49/50,L6,L19 and o-aminobenzenaldehyde,which might be the basis of pharmacodynamic substances.Conclusion:There were many ways of transformation of qualitative and quantitative changes of characteristic components after processing,and these transformation ways may be the detoxifi

关 键 词：高乌头 甘草汁 模拟炮制 高乌甲素 冉乌头碱 减毒存效 UPLC-Q-TOF-MS 转化机制 

分 类 号：R284.1[医药卫生—中药学]