不同pH对菠萝幼苗根系生长和叶片生理特性的影响  

Effect of Different pH on Root Growth and Leaf Physiological Characteristics of Pineapple Seedlings

作　　者：丁京晶 罗嘉腾 黄家权[1] Ding Jingjing;Luo Jiateng;Huang Jiaquan(College of Tropical Crops,Hainan University,Haikou,570000)

机构地区：[1]海南大学热带作物学院,海口570000

出　　处：《分子植物育种》2024年第1期222-229,共8页Molecular Plant Breeding

基　　金：海南省高层次人才项目(321RC1027)资助。

摘　　要：本试验以‘金菠萝’为材料,探究了水培条件下不同pH值(3~9)对菠萝幼苗根系及叶片生理特性的影响。结果表明,随着pH值的升高,最长根长、根量、根鲜重和苗鲜重均逐渐降低,表明菠萝幼苗的生根及地上部生长在越高的pH条件下受到的抑制作用越明显;不同pH处理对菠萝幼苗叶片的叶绿素含量无显著差异,菠萝幼苗叶片的类胡萝卜素含量在pH=9条件下显著低于pH=3处理;叶片膜透性在pH值为8、9条件下显著高于pH=3处理;在pH值为7、8、9处理条件下,菠萝幼苗叶片的可溶性蛋白含量显著高于pH=3处理;不同pH处理下菠萝幼苗叶片的可溶性糖含量与脯氨酸含量无显著差异;pH值为8、9时水培后的菠萝幼苗丙二醛含量显著高于pH=3处理;不同pH处理对超氧化物歧化酶活性(SOD活性)没有产生明显影响;pH值为8和9时菠萝幼苗的过氧化物酶活性(POD活性)显著高于pH=3处理;pH=9时菠萝幼苗体内过氧化氢酶活性(CAT活性)显著高于pH值为3、4处理;随pH的变化,抗坏血酸过氧化物酶活性(APX活性)刚开始整体变化不大,pH=9时,APX活性显著升高,其他不同pH处理间无显著差异。由此可知,较低的pH值(3,4)有利于菠萝幼苗的生长,而较高的pH值(8,9)则抑制菠萝根部生长及其地上部的生长。In this experiment,the effects of different pH values(pH=3~9) were investigated on the physiological characteristics of roots and leaves of pineapple seedlings under hydroponic conditions using golden pineapple as the material.The results showed that the longest root length,root volume,root fresh weight and seedling fresh weight were decreased with increasing pH,indicating the inhibition of rooting and above-ground growth of pineapple seedlings at higher pH conditions.The chlorophyll content of pineapple seedling leaves were not significantly different between the different pH treatments.The carotenoid content of pineapple seedling leaves were significantly lower at pH=9 than at pH=3.The membrane permeability of pineapple seedling leaves were significantly higher at pH=8 and pH=9 than at pH=3.The soluble protein content of pineapple seedling leaves were significantly higher at pH=7,8,and 9 than at pH=3.The soluble sugar content and proline content of pineapple seedling leaves were not significantly different between the different pH treatments.The malondialdehyde content and peroxidase(POD) activity of pineapple seedlings were significantly higher at higher pH(pH=8 and 9) than at lower pH(pH=3).The superoxide dismutase(SOD) activity was not significantly affected by the different pH treatments.The catalase(CAT) activity of pineapple seedlings were significantly higher at higher pH(pH=9) than at lower pH(pH=3 and 4).The overall change in ascorbate peroxidase activity(APX) was not significant at the beginning and increased significantly at pH=9.There were no significant differences between the other pH treatments.In conclusion,the lower pH(pH=3 and 4) favored the growth of pineapple seedlings,while higher pH(pH=8 and 9)inhibited the above-ground and root growth of pineapple.

关 键 词：菠萝 PH 根系生长 生理特性 

分 类 号：S668.3[农业科学—果树学]