黄芩苷对支原体肺炎小鼠TLR4/NF-κB信号通路的抗炎及肺功能保护作用  

Effects of baicalin on the anti-inflammatory and pulmonary function of TLR4/NF-κB signaling pathway in mice with Mycoplasma pneumoniae

作　　者：吴文娟 曾妮[1] 王硕莹 孙京焕 黄少祥[1] WU Wen-juan;ZENG Ni;WANG Shuo-ying;SUN Jing-huan;HUANG Shao-xiang(Tianjin Fifth Central Hospital,Tianjin 300450,China)

机构地区：[1]天津市第五中心医院呼吸科,天津300450

出　　处：《中华医院感染学杂志》2023年第23期3521-3526,共6页Chinese Journal of Nosocomiology

基　　金：天津市卫生局科技基金资助项目(2014KZ019)。

摘　　要：目的探讨黄芩苷对支原体肺炎小鼠Toll样受体4/核因子κB(TLR4/NF-κB)信号通路及炎性反应的影响。方法60只BALB/c小鼠按照随机数字表法分为对照组、模型组、阿奇霉素组和黄芩苷高、中、低剂量组。采用滴鼻法建立支原体肺炎感染小鼠模型,采用黄芩苷和阿奇霉素进行干预,检测基本体征、肺功能、肺组织病理学变化、炎性因子水平,以及肺组织中鼠TLR4、NF-κB相关mRNA转录水平及蛋白相对表达水平。结果与对照组相比,模型组、阿奇霉素组、黄芩苷高剂量组、黄芩苷中剂量组、黄芩苷低剂量组小鼠体温、肺指数高,体质量、肺干湿比低(P<0.05);与模型组比较,阿奇霉素组与黄芩苷高、中、低剂量组的呼气峰值流速(PEF)、最大通气量(MVV)、动态肺顺应性(Cydn)上调,吸气阻力(RI)、呼气阻力(RE)、肺总阻力(RL)下调,肺功能得到保护,且肺泡灌洗液、血清、肺组织中炎性因子水平下调;阿奇霉素组与黄芩苷高、中、低剂量组肺组织病理损伤程度降低,与模型组比较,阿奇霉素组与黄芩苷高、中、低剂量组TLR4、NF-κB、人核因子κB抑制蛋白α(I-κBα)的mRNA转录水平及蛋白相对表达水平下调(P<0.05)。结论黄芩苷可能通过调节TLR4/NF-κB信号通路表达,抑制炎性反应来实现保护肺功能、抗炎的作用。OBJECTIVE To explore the effects of baicalin on the Toll-like receptors 4/nuclear factor κB(TLR4/NF-κB)signaling pathway and inflammatory response in mice with Mycoplasma pneumoniae.METHODS A total of 60 BALB/c mice were divided into the control group,model group,azithromycin group,baicalin high dose group,baicalin medium dose group and baicalin low dose group by using random numerical table method.Mouse model of Mycoplasma pneumoniae infection was established by intranasal method,and baicalin and azithromycin were used for the intervention.Basic signs,lung function,pulmonary histopathological changes and inflammatory factor levels,TLR4 and NF-κB-related mRNA and protein expression levels in lung tissues of the mice were detected.RESULTS Compared with the control group,the body temperature and lung index were higher than those in the model group,the azithromycin group,the baicalin high-dose group,the baicalin medium-dose group and the baicalin low-dose group,while the body mass,and lung dry-to-wet ratio were lower(P<0.05).Compared with the model group,the peak exhalation flow(PEF),maximal voluntary ventilation(MVV),and dynamic lung compliance(Cydn)in the azithromycin group and the baicalin high-dose,medium-dose,and low-dose groups were upregulated,while inspiratory resistance(RI),exhalation resistance(RE)and lung resistance(RL)were downregulated.Lung function was protected,and the levels of inflammatory factors in alveolar lavage fluid,serum,and lung tissue were downregulated.The degree of pathological damage to lung tissue in the azithromycin group and baicalin high-dose,medium-dose,and low-dose groups decreased.When compared with the model group,the mRNA and protein relative expression levels of TLR4,NF-κB,and human nuclear factorκB inhibitory protein α(I-κB)were downregulated in the azithromycin group and baicalin high-dose,medium-dose,and low-dose groups(P<0.05).CONCLUSION Baicalin may inhibit inflammatory response by regulating the expression of TLR4/NF-κB signaling pathway,thereby protecting lung fu

关 键 词：黄芩苷 支原体肺炎 小鼠 肺功能 抗炎 炎症反应 

分 类 号：R375[医药卫生—病原生物学]