缝隙增强拉曼探针:制备、光学属性和应用  

Gap-enhanced Raman tags(GERTs):synthesis,optical properties and applications

作　　者：邓斌格 林俐 叶坚 DENG Binge;LIN Li;YE Jian(School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200030,China)

机构地区：[1]上海交通大学生物医学工程学院,上海200030

出　　处：《光散射学报》2023年第4期311-325,共15页The Journal of Light Scattering

基　　金：国家自然科学基金(No.82272054)。

摘　　要：缝隙增强拉曼探针(Gap-enhanced Raman tags,GERTs)是一种基于表面增强拉曼散射(Surface-enhanced Raman spectroscopy,SERS)技术的新型核壳纳米光学探针。SERS是一种非常敏感的光谱技术,用于检测分子的振动和转动信息。在SERS中,当分子与金属纳米结构(如金、银纳米颗粒)接触时,会产生强烈的拉曼散射信号增强效应,可以使分子的拉曼散射信号增加10个数量级以上。GERTs探针通过将拉曼报告分子包埋在颗粒内部缝隙中,获得超高的检测灵敏度,可达到单颗粒检测水平,同时,其具有分子特征光谱、特异性高,因此被广泛应用于各个领域。本文介绍了GERTs的制备和光学属性,以实现满足于实际应用的高拉曼性能的GERTs探针。同时,我们对GERTs首次作为物理不可克隆标签的应用,以及其在生物医学成像和综合诊疗方面的最新进展进行了全面综述。最后,我们对GERTs在真实应用场景中所面临的挑战进行了全面评估,为推动GERTs技术的临床转化提供了重要的参考依据。展望未来,随着进一步的研究和发展,新型、稳定且高亮度的GERTs探针定将成为一项关键的分析工具,为实际应用和科学研究提供更为丰富的可能性。Gap-enhanced Raman tags(GERTs)represent an innovative optical probe designed based on surface-enhanced Raman scattering(SERS).SERS is a highly sensitive spectroscopic technique used to detect molecular vibrational and transitional information.In the context of SERS,the interaction between molecules and metallic nanostructures,such as Au or Ag nanoparticles,leads to a significant enhancement of Raman scattering signals—a phenomenon capable of amplifying molecular Raman scattering signals by over 10 orders of magnitude.By embedding Raman reporter molecules inside the internal plasmonic metallic nano-junction,GERTs exhibit extraordinary detection sensitivity down to the single-particle detection level;coupled with their ability to provide high-resolution molecular features,GERTs have found extensive applications across a multitude of fields.In this article,we provide a introduction to the preparation and optical properties of GERTs,with the goal of realizing their use in practical applications.At the same time,we provide a review of the pioneering use of GERTs as physically unclonable tags,along with their recent advances in biomedical imaging and integrated diagnostics.Finally,we provide a assessment of the challenges GERTs face in real-world application scenarios,providing critical reference points for advancing the clinical translation of GERTs technology.Looking ahead,with further research and development,the novel,stable and super-bright GERTs probes are poised to become a central analytical tool,offering even richer opportunities for practical applications and scientific research.

关 键 词：表面增强拉曼光谱 纳米探针 缝隙增强拉曼探针 生物检测 医学成像 

分 类 号：O657[理学—分析化学]