Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA(PbA)infection in mice  

作　　者：Mohammad Faruq Abd Rachman Isnadi Rusliza Basir Ramatu Bello Omenesa Roslaini Abd Majid Maizaton Atmadini Abdullah Che Norma Mat Taib Sivan Padma Priya Yong Yean Kong Chin Voon Kin Gambo Lawal Mukhtar 

机构地区：[1]Department of Human Anatomy,Faculty of Medicine&Health Sciences,Universiti Putra Malaysia,Serdang,Selangor,Malaysia [2]Department of Pharmacology and Therapeutics,Faculty of Pharmaceutical Sciences,Ahmadu Bello University,Zaria,Kaduna,Nigeria [3]Faculty of Medicine and Defence Health,National Defence University of Malaysia,Kem Sungai Besi,Kuala Lumpur,Malaysia [4]Department of Pathology,Faculty of Medicine&Health Sciences,Universiti Putra Malaysia,Serdang,Selangor,Malaysia [5]RAK College of Dental Sciences,Ras Al Khaimah Medical and Health Sciences University,Ras Al Khaimah,United Arab Emirates [6]Laboratory Centre,Xiamen University Malaysia,Sepang,Selangor,Malaysia [7]Segi University,Faculty of Medicine,Nursing and Health Sciences,9,Jalan Tecknologi,PJu 5 Kota Damansara,Petaling,Selangor,Malaysia [8]Department of Pathobiology and Medical Diagnostics,Faculty of Medicine and Health Sciences,Universiti Malaysia Sabah,Kota Kinabalu,Malaysia [9]Department of Microbiology,Faculty of Natural&Applied Sciences,Umaru Musa Yar’adua University,Katsina,Nigeria

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2023年第12期521-531,I0004,I0005,共13页亚太热带生物医学杂志（英文版）

基　　金：supported by the Fundamental Research Grant Scheme(FRGS)from the Malaysia Ministry of Higher Education(FRGS/1/2016/SKK10/UPM/02/1).

摘　　要：Objective:To determine the involvement and the modulatory effects of IL-33 during Plasmodium berghei ANKA(PbA)infection.Methods:PbA infection in male ICR mice was utilized as a model of malaria.Systemically circulating IL-33 levels were determined in blood plasma by enzyme-linked immunosorbent assay(ELISA).After 24 hours post-inoculation of PbA,recombinant IL-33 and ST2,and antibodies against IL-33 and IgG treatments were administered daily for 3 days.Tissue expression and localization of IL-33 were assessed in organs generally affected by malaria via immunohistochemistry.Moreover,histopathological examination was performed to assess the effects of the treatments.Results:The levels of systemic IL-33 were elevated at the critical phase of PbA infection.Likewise,immunohistochemical analysis revealed a significant upregulation of IL-33 expression at the critical phase in the brain,lungs,and spleen of PbA-infected mice as compared to healthy controls.Treatment with IL-33 protected against experimental cerebral malaria development and reduced pathological features in the brain and lungs of the PbA-infected mice.Conclusions:A potential critical role and involvement of IL-33 in PbA infection may hint at the resolution of immunopathological sequelae associated with malaria.

关 键 词：Plasmodium berghei ANKA Malaria IL-33 IMMUNOLOGY Immunotherapy 

分 类 号：R285.5[医药卫生—中药学]