亚高山粗枝云杉人工林土壤原核微生物群落结构与功能变化  

Changes in the structure and function of soil prokaryotic communities in subalpine Picea asperata plantations

作　　者：刘艳娇 刘庆[1] 贺合亮 赵文强 寇涌苹 LIU Yanjiao;LIU Qing;HE Heliang;ZHAO Wenqiang;KOU Yongping(Chengdu Institute of Biology,Chinese Academy of Sciences/CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization/Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province,Chengdu 610041,China;College of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Yibin University,Yibin 644007,Sichuan,China)

机构地区：[1]中国科学院成都生物研究所/中国科学院山地生态恢复与生物资源利用重点实验室/生态恢复与生物多样性保育四川省重点实验室,成都610041 [2]福建农林大学资源与环境学院,福州350002 [3]宜宾学院,四川宜宾644007

出　　处：《应用生态学报》2023年第12期3279-3290,共12页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金面上项目(32171550);中国科学院青年创新促进会项目(2021371)资助。

摘　　要：土壤原核微生物群落结构和功能特性对维持生态系统功能至关重要。本研究以不同林龄粗枝云杉为研究对象,结合高通量测序技术和生物信息学方法,研究粗枝云杉根际和非根际土壤原核微生物群落多样性和群落组成变化特征及其关键影响因素,并分析原核微生物群落功能特征的变化。结果表明:土壤原核微生物群落的β多样性在根际与非根际均表现为不同林龄之间差异显著,而同一林龄的根际与非根际间无显著差异。从群落组成来看,门水平上,变形菌门和己科河菌门的相对丰度随人工林林龄增加而升高,而放线菌门随林龄增加而降低,且75年人工林(PF75)与天然林(NF)之间无显著差异。厚壁菌门和奇古菌门的相对丰度在25年云杉林(PF25)土壤中显著高于其他人工林和NF。属水平上,RB41、Terrimonas和酸杆菌属的相对丰度随人工林林龄增加而升高,且根际土壤中的RB41和Terrimonas在PF75显著高于NF。土壤理化性质和植被特性共同影响了原核微生物的群落结构,其中,草本覆盖度、土壤pH、总磷和总氮是主要影响因素。原核微生物群落的功能特性在不同林龄之间具有显著差异。与碳氮循环相关的部分功能(如纤维素降解和硝化作用)的相对丰度随人工林林龄增加而降低,而与硫循环相关的硫酸盐呼吸功能的相对丰度则升高。建议将土壤原核微生物群落的结构和功能特征作为云杉林发育阶段的重要指示。在人工林发育后期可通过调控解磷增氮的微生物以提高土壤养分有效性来维持人工林生态系统稳定。The structural and functional characteristics of soil prokaryotic community are important for maintaining ecosystem functions. In this study, we examined the diversity and compositions, the key drivers, as well as functional characteristics of prokaryotic communities in the rhizosphere and non-rhizosphere soils of Picea asperata with different stand ages using high-throughput sequencing technique and bioinformatics methods. The results showed that β-diversity of soil prokaryotic communities in both rhizosphere and non-rhizosphere showed significant differences among different stand ages, but no significant difference between rhizosphere and non-rhizosphere in the same stand age. In terms of community composition at the phylum level, the relative abundances of Proteobacteria and Rokubacteria showed an increasing trend with the increases of stand age, while the relative abundance of Actinobacteria showed a decreasing trend, but no significant difference was observed between 75 year-old planted forests(PF75) and natural forests(NF). The relative abundances of Firmicutes and Thaumarchaeota in the soil of the 25 year-old planted forests(PF25) were significantly higher than in other planted forests and NF. At the genus level, the relative abundances of RB41, Terrimonas and Acidibacter showed an increasing trend with the increases of stand age, and RB41 and Terrimonas in rhizosphere soil of PF75 were significantly higher than those in NF. Soil properties and vegetation characteristics jointly influenced the structure of soil prokaryotic communities, with herb layer coverage, soil pH, total phosphorus, and total nitrogen as major drivers. The functional characteristics of soil prokaryotic communities were significantly different among different stand ages. The relative abundances of functions involved in carbon and nitrogen cycle, e.g., cellulolysis and nitrification, decreased with the increases of stand age, whereas that of sulfate respiration involved in the sulfur cycle increased. We proposed that the structure and

关 键 词：亚高山森林 人工林 粗枝云杉 土壤原核微生物 高通量测序 功能预测 

分 类 号：S714.3[农业科学—林学]