甲基化SIM2、GNA12、CTGF在子痫前期孕妇中表达水平及其对疾病的预测价值  

Expression levels of methylated SIM2,GNA12 and CTGF in pregnant women with preeclampsia and its predictive value for disease

作　　者：张阔 陈莹[1] 刘丹[1] 汪俊红[1] ZHANG Kuo;CHEN Ying;LIU Dan;WANG Junhong(Department of Obstetrics,Tangshan Maternal and Child Health Hospital,Tangshan,Hebei 063000,China)

机构地区：[1]唐山市妇幼保健院产科,河北唐山063000

出　　处：《国际检验医学杂志》2024年第1期44-48,共5页International Journal of Laboratory Medicine

基　　金：河北省医学科学研究课题计划项目(20191527)。

摘　　要：目的 探究子痫前期孕妇血浆中甲基化DNA的表达水平及对子痫前期发生的预测价值。方法 纳入2022年1-12月在该院确诊的82例子痫前期孕妇作为观察组,另外纳入82例健康孕妇作为对照组。提取患者游离总DNA,经过DNA亚硫酸氢盐修饰后通过实时荧光定量PCR反应(qRT-PCR)检测患者血浆中甲基化单意同源物2(SIM2)、结缔组织生长因子(CTGF)及鸟嘌呤核苷酸结合蛋白(GNA12)基因的相对表达水平,并采用相关性分析及受试者工作特征(ROC)曲线对各甲基化DNA预测子痫前期发生的价值进行评估。结果 观察组血浆甲基化SIM2、GNA12、CTGF相对表达水平均高于对照组(P<0.05),且重度子痫前期孕妇各甲基化DNA相对表达水平更高(P<0.05)。相关性分析结果表明,血浆甲基化SIM2、GNA12、CTGF相对表达水平与孕妇发生子痫前期均呈正相关(P<0.05)。ROC曲线分析结果表明,血浆甲基化SIM2、GNA12、CTGF相对表达水平单独及联合检测对预测孕妇子痫前期的效能均较好,且三者联合检测的预测效能最高(曲线下面积为0.888,95%CI:0.827~0.949)。结论 相较于健康孕妇,子痫前期孕妇血浆中甲基化SIM2、GNA12、CTGF相对表达水平均较高,且其与孕妇子痫前期的发生率呈正相关,血浆甲基化SIM2、GNA12及CTGF相对表达水平有望成为判断子痫前期是否发生的重要指标。Objective To investigate the plasma levels of methylated DNA in the pregnant women with preeclampsia and its predictive value for the occurrence of preeclampsia.Methods A total of 82 pregnant women with preeclampsia admitted to the hospital from January to December 2022 were included as the observation group,and another 82 healthy pregnant women were included as the control group.Total DNA was extracted,and the relative expression levels of methylated single-intention homolog 2(SIM2),guanine nucleotide-binding protein(GNA12),and connective tissue growth factor(CTGF)genes in plasma were detected by real-time fluorescence quantitative PCR(qRT-PCR)after DNA bisulfite modification.The value of methylated DNA in predicting preeclampsia was evaluated by correlation analysis and receiver operating characteristic(ROC)curve.Results The relative expression levels of methylated SIM2,GNA12 and CTGF in plasma in the observation group were significantly higher than those in the control group(P<0.05),and the relative expression levels of methylated SIM2,GNA12 and CTGF in severe preeclampsia group was higher(P<0.05).Correlation analysis showed that the relative expression levels of methylated SIM2,GNA12 and CTGF in plasma were significantly positively correlated with the occurrence of preeclampsia in pregnant women(P<0.05).ROC curve analysis results showed that the relative expression levels of plasma methylation SIM2,GNA12,and CTGF,both individually and in combination,had good predictive efficacy in predicting preeclampsia in pregnant women,and the combined detection of the three had the highest predictive efficacy(area under the curve was 0.888,95%CI:0.827-0.949).Conclusion Compared with healthy pregnant women,the relative expression levels of methylated SIM2,GNA12 and CTGF in plasma are higher in pregnant women with preeclampsia,which are positively correlated with the occurrence of preeclampsia and the severity of the disease.The relative expression levels of methylated SIM2,GNA12 and CTGF are expected to be important predic

关 键 词：子痫前期 甲基化DNA 单意同源物2 鸟嘌呤核苷酸结合蛋白 结缔组织生长因子 

分 类 号：R446.9[医药卫生—诊断学]