性别差异与颈动脉粥样硬化斑块:基于UPLC-MS技术的代谢组学研究  

Gender differences with carotid atherosclerotic plaque:a metabolomic study based on UPLC-MS

作　　者：喻恬 张秋娟 鲁鹤臻 王优 徐保锋[3] 刘锐[1] YU Tian;ZHANG Qiujuan;LU Hezhen(Department of Very Important People(VIP)Unit,China-Japan Union Hospital of Jilin University,Changchun 130033,China)

机构地区：[1]吉林大学中日联谊医院VIP科,吉林长春130033 [2]吉林大学中日联谊医院检验科,吉林长春130033 [3]吉林大学白求恩第一医院卒中中心,吉林长春130021

出　　处：《中风与神经疾病杂志》2023年第12期1108-1113,共6页Journal of Apoplexy and Nervous Diseases

基　　金：吉林大学研究生创新基金(202209)。

摘　　要：目的本研究探讨不同性别颈动脉粥样硬化斑块患者尿液代谢产物差异,探索颈动脉粥样硬化斑块在男女患者中的不同发病机制,尝试解释性别导致的生物学差异。方法收集患有颈动脉粥样硬化斑块的28例男性、14例女性患者的尿液,通过(ultra performance liquid chromatography-mass spectrometry,UPLC-MS)技术进行代谢组学分析。对获取到的代谢组学数据进行基于Metaboanalyst网站的数据分析,从而鉴定男性和女性患者之间的差异代谢物。为了进一步了解代谢物的功能,对代谢物进行生物学功能和通路富集分析。结果颈动脉粥样硬化斑块不同性别的患者的尿液中,34个代谢物显著差异表达。这些代谢物的功能主要富集在组氨酸代谢、β-丙氨酸代谢、精氨酸生物合成、泛酸和辅酶A生物合成、谷胱甘肽代谢通路上。结论男性和女性组颈动脉粥样硬化斑块患者的尿液代谢谱存在显著差异。Objective The purpose of this study is to explore the differences of urine metabolites in carotid atherosclerotic plaque patients of different sexes,explore the different pathogenesis of carotid atherosclerotic plaque in men and women,and try to explain the biological differences caused by gender.Methods Urine of 28 men and 14 women with carotid atherosclerotic plaque were collected and analyzed by ultraperformance liquid chromatography mass spectrometry(UPLC-MS).Perform metabolomics data analysis based on the Metaboanalysis website to identify differential metabolites between male and female patients.In order to further understand the function of metabolites,biological function and pathway enrichment analysis were conducted on metabolites.Results There were significant differences in the expression of 34 metabolites in the urine of patients with carotid atherosclerotic plaque of different sexes.The functions of these metabolites are mainly enriched in histidine metabolism,β-Alanine metabolism,arginine biosynthesis,pantothenate and coenzyme A biosynthesis,and glutathione metabolism pathways.Conclusion There are significant differences in urine metabolic profiles between male and female patients with carotid atherosclerotic plaque.

关 键 词：颈动脉粥样硬化斑块 代谢组学 男性 女性 

分 类 号：R543.4[医药卫生—心血管疾病]