氯化亚铁溶液建立大鼠外伤性癫痫的造模方法及高压氧治疗研究  

FeCl_(2)Modeling Method and Hyperbaric Oxygen Therapy for Posttraumatic Epilepsy in Rats

作　　者：周文[1] 陈史芳[2] 杨宇 谢泽宇[1] Zhou Wen;Chen Shifang;Yang Yu;Zeyu Xie(The Second Affiliated Hospital,Medical College of Shantou University,Shantou 515041;Shantou Central Hospital Medical Oncology,Shantou 515041;South China Agricultural University,Guangzhou 510640,China)

机构地区：[1]汕头大学医学院第二附属医院,广东汕头515041 [2]汕头市中心医院,广东汕头515041 [3]华南农业大学,广东广州510640

出　　处：《广东化工》2023年第24期60-62,72,共4页Guangdong Chemical Industry

摘　　要：目的:皮层注射氯化亚铁溶液建立外伤性癫痫大鼠模型及高压氧治疗对其防治及作用机制。方法:采用大脑皮层注射氯化亚铁建立外伤性癫痫大鼠模型,分模型组、治疗组及正常组进行行为学观察及脑电图监测,取脑组织观察神经细胞形态改变,检测各组样本c-fos mRNA、c-jun mRNA、c-myc mRNA的表达水平。结果:皮层注射氯化亚铁建立外伤性癫痫大鼠模型,操作简单,成功率高,稳定可靠,是值得推广的全新造模方法。该模型中c-fos、c-jun、c-myc均呈现长时程表达。结论:皮层注射氯化亚铁可通过诱导神经细胞凋亡导致癫痫发作。高压氧治疗外伤性癫痫可通过阻断c-jun、c-myc基因介导的细胞凋亡通路而起到治疗效果。Objective:Methods of FeCl_(2)modeling in rats with posttraumatic epilepsy and the prevention and mechanism of action of hyperbaric oxygen therapy.Methods:A rat model of posttraumatic epilepsy was established by injecting FeCl_(2)into the cerebral cortex,and the model group,treatment group and normal group were divided for behavioral observation and electroencephalogram monitoring,and brain tissues were taken to observe the morphologic changes of neuronal cells,and to detect the expression levels of c-fos mRNA,c-jun mRNA,and c-myc mRNA in samples from each group.Results:Cortical injection of FeCl_(2)to establish a rat model of posttraumatic epilepsy is simple,successful,stable and reliable,and is a new modeling method worth promoting.In this model,c-fos,c-jun and c-myc showed long time-course expression.Conclusion:Cortical injections of FeCl_(2)may cause seizures by inducing apoptosis in neuronal cells.Hyperbaric oxygen therapy for traumatic epilepsy can be therapeutic by blocking the c-jun and c-myc gene-mediated apoptotic pathway.

关 键 词：氯化亚铁 外伤性癫痫 高压氧 造模 早期快反应基因 

分 类 号：TQ[化学工程]