子宫内膜异位症患者在位及异位内膜上皮细胞-间充质转化相关生物标志物的变化  

Differences in epithelial mesenchymal transition-related biological markers between in-situ and ectopic endometrial epithelial cells in endometriosis patients

作　　者：罗丹 孔为民[1] 陈姝宁 赵小玲 谢云凯 Luo Dan;Kong Weimin;Chen Shuning;Zhao Xiaoling;Xie Yunkai(Department of Gynecology,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing Maternal and Child Health Care Hospital,Beijing 100006,China)

机构地区：[1]首都医科大学附属北京妇产医院/北京妇幼保健院妇科,100020

出　　处：《中华妇幼临床医学杂志（电子版）》2023年第5期530-539,共10页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：国家自然科学基金项目(81871142)。

摘　　要：目的探讨子宫内膜异位症(EMS)患者在位及异位内膜上皮细胞-间充质转化(EMT)相关生物标志物的表达特点。方法在R语言GEOquery包的基因表达总览(GEO)数据库中,下载EMS相关数据集GSE7305。该数据集来源于10例EMS患者的异位子宫内膜与10例正常子宫内膜受试者的子宫内膜组织,采用生物信息学方法分析该数据集中,EMT相关差异基因表达情况。选择2020年10月1日至2022年3月31日首都医科大学附属北京妇产医院收治的采取全子宫切除术(6例)、经腹腔镜患侧卵巢囊肿剔除术+宫腔镜检查(8例)、经腹腔镜患侧卵巢囊肿剔除术(13例)治疗的27例EMS患者,以及采取全子宫切除术治疗的14例宫颈上皮内瘤变(CIN)3与宫颈癌ⅠA1期患者为研究对象。根据患者术后活组织病理学诊断结果,将其分别纳入EMS异位内膜组(n=27,所有EMS患者的异位子宫内膜),EMS在位内膜组(n=14,采取全子宫切除术、经腹腔镜患侧卵巢囊肿剔除术+宫腔镜检查治疗的EMS患者的在位子宫内膜),以及对照组(n=14,CIN3与宫颈癌ⅠA1期患者的正常子宫内膜)。再根据获取EMS异位内膜组患者子宫内膜时所处月经周期,将其进一步分为EMS异位内膜增生期亚组(n=14)与EMS异位内膜分泌期亚组(n=13)。采用免疫组织化学(IHC)方法检测EMS异位内膜组、EMS在位内膜组与对照组患者子宫内膜组织EMT相关生物标志物,如E-钙黏着蛋白(E-cadherin)、N-钙黏着蛋白(N-cadherin)、波形蛋白(vimentin)及snail家族(snail、slug)与碱性螺旋-环-螺旋家族twist转录因子表达情况,这些生物标志物阳性表达率与组织化学评分(H-score)值比较,采用χ^(2)检验、Fisher确切概率法与Kruskal-Wallis H检验,进一步两两比较采用Bonferroni法校正检验水准。本研究遵行的程序符合首都医科大学附属北京妇产医院伦理委员会规定,并获得该伦理委员会批准(审批文号:2019-KY-002-01),所有患者均签署临床研究Objective To investigate the expression characteristics of epithelial mesenchymal transition(EMT)-related biological markers in in-situ and ectopic endometrium of endometriosis(EMS)patients.Methods The EMS-related dataset GSE7305 was downloaded from the Gene Expression Omnibus(GEO)database via the GEOquery package in R language.The dataset was derived from ectopic endometrial tissues of 10 EMS patients and in-situ endometrial tissues of 10 subjects with normal endometrial tissues.EMT-related differential gene expression in this dataset was analyzed by bioinformatics method.A total of 27 EMS patients who received total hysterectomy(6 cases)or laparoscopic oophorocystectomy of affected side and hysteroscopy(8 cases)or laparoscopic oophorocystectomy of affected side(13 cases)and 14 patients with cervical intraepithelial neoplasia(CIN)3 and cervical carcinoma stageⅠA1 who underwent total hysterectomy in Beijing Obstetrics and Gynecology Hospital,Capital Medical University from October 1,2020 to March 31,2022 were selected as study subjects.The patients were included in EMS ectopic endometrium group(n=27,ectopic endometrium in all EMS patients),EMS in-situ endometrium group(n=14,in-situ endometrium in EMS patients who received total hysterectomy or laparoscopic oophorocystectomy of affected side and hysteroscopy),and control group(n=14,normal endometrium in patients with CIN3 and cervical carcinoma stageⅠA1),according to the diagnostic results of postoperative biopsy histopathology.The patients in EMS ectopic endometrium group were further divided into EMS ectopic endometrium proliferative subgroup(n=14)and EMS ectopic endometrium secretory subgroup(n=13)according to the menstrual cycle of patients in EMS ectopic endometrium group during which the endometrium was obtained.Immunohistochemistry(IHC)was used to detect the expression of EMT-related markers,such as E-cadherin,N-cadherin,vimentin,snail family(snail,slug)and twist transcription factors in endometrial tissues of patients in EMS ectopic endometrium group,E

关 键 词：子宫内膜异位症 上皮细胞-间充质转化 生物标志物 差异基因 子宫内膜异位症在位与异位子宫内膜 妇女 

分 类 号：R711.71[医药卫生—妇产科学]