防风总色原酮苷元提取工艺优化及其抗炎活性研究  

Studies on Optimization of Extraction Process and Anti-inflammatory Activity of Total Chromone Aglycones in Saposhnikovia divaricata

作　　者：陈浩 李艳露 韩佳宏[1] 宋佳琪 韩梅[1] 蔡恩博[1] 杨利民[1] Chen Hao;Li Yanlu;Han Jiahong;Song Jiaqi;Han Mei;Cai Enbo;Yang Limin(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《中国野生植物资源》2023年第12期30-36,共7页Chinese Wild Plant Resources

基　　金：吉林省科技发展计划项目(20210101217JC)。

摘　　要：目的:优化防风总色原酮苷元提取工艺,并初步探究其体外抗炎活性。方法:以升麻素及5-O-甲基维斯阿米醇总提取率为指标,通过响应面法优化防风总色原酮苷元提取工艺;采用脂多糖(LPS)诱导的小鼠腹腔巨噬细胞(RAW 264.7)炎症模型,探究最优参数下防风总色原酮苷元对细胞上清中一氧化氮(NO)含量的影响。结果:最佳工艺为加酶量89.87%,酶解时间59.97 min,酶解温度55.95℃;预测提取率为4.58 mg/g,实际平均提取率为4.49 mg/g,与理论值相差0.09 mg/g,与未加酶提取法相比,提高了3倍;最优参数下防风总色原酮苷元显著抑制了LPS诱导的RAW 264.7细胞NO分泌,与参比提取物比较,抑制效果显著提高,具有良好的体外抗炎活性。结论:该工艺稳定性好,提取率高,提取物抗炎活性好,为防风药材的开发利用提供了新思路。Objective:To optimize the extraction conditions of total chromone aglycones in Saposhnikoviae Radix and study its anti-inflammatory activity in vitro.Methods:The extraction process of total chromone aglycones from Saposhnikoviae Radix was optimized by response surface method with the total extraction rate of cimifugin and 5-O-methylvisamminol as indexes.The inflammatory model of mouse peritoneal macrophages(RAW 264.7)induced by lipopolysaccharide(LPS)was used to study the effect of total chromone aglycones of Saposhnikoviae Radix on the content of nitric oxide(NO)in cell supernatant under the optimal parameters.Results:The optimum extraction conditions were as follows:the amount of enzyme 89.87%,enzymolysis time 59.97 min,enzymolysis temperature 55.95℃.The predicted extraction rate was 4.58 mg/g,and the actual average extraction rate was 4.49 mg/g,which was 0.09 mg/g different from the theoretical value.Under the optimal parameters,the total chromogen ketone aglycones of Saposhnikoviae Radix significantly inhibited LPS-induced NO secretion in RAW 264.7 cells.Compared with the reference extract,the inhibitory effect was significantly improved and had good anti-inflammatory activity in vitro.Conclusion:The process had good stability,high extraction rate and good anti-inflammatory activity,which provided a new idea for the development and utilization of Saposhnikoviae Radix.

关 键 词：防风 色原酮苷元 纤维二糖酶 提取工艺 响应面法 

分 类 号：R284.2[医药卫生—中药学]