同位素稀释-超高效液相色谱-串联质谱法测定化妆品中大麻二酚和四氢大麻酚  

Determination of Cannabidiol and Tetrahydrocannabinol in Cosmetics Using Isotope Dilution Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：李长于 胡丹 石瑞瑞 杨铭 鹿燕 廖上富 LI Changyu;HU Dan;SHI Ruirui;YANG Ming;LU Yan;LIAO Shangfu(Zhejiang FangYuan Test Group Co.,Ltd,Hangzhou 310018)

机构地区：[1]浙江方圆检测集团股份有限公司,浙江杭州310018

出　　处：《分析科学学报》2023年第6期711-715,共5页Journal of Analytical Science

基　　金：浙江省市场监督管理局雏鹰计划培育项目(项目编号:CY2022005)资助。

摘　　要：建立了超高效液相色谱-串联质谱(UPLC-MS/MS)测定化妆品中大麻二酚(Cannabidiol,CBD)和四氢大麻酚(Tetrahydrocannabinol,THC)的分析方法。对水剂、乳液、膏霜、面膜、口红及蜜粉6种基质类样品,利用甲醇超声提取,提取液经离心后得到样品溶液,以水-甲醇为流动相,选择Acquity UPLC BEH C18色谱柱(100 mm×2.1 mm,1.7μm)进行梯度分离。采用电喷雾(ESI)负离子电离模式和多反应监测(MRM)扫描模式,同位素(四氢大麻酚-D3,THC-D3)内标法定量。结果显示,CBD和THC在1.0~20 ng/mL质量浓度范围内均呈良好线性关系,相关系数(R^(2))均大于0.995,检出限(S/N=3)分别为0.5、0.2μg/kg,6种基质样品的加标回收率为81.3%~109.5%,相对标准偏差为1.7%~11.4%。该方法操作简单、快速且灵敏度高,适用于化妆品中大麻二酚和四氢大麻酚的测定。A method for the determination of Cannabidiol and Tetrahydrocannabinol in cosmetics was established by using isotope dilution ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Six kinds of matrix samples,including water-based,lotion,cream,facial mask,lipstick and powder were ultrasonically extracted with methanol,then the sample solution was prepared after centrifugation,and the analytes were separated on an Acquity UPLC BEH C18 column(100 mm×2.1 mm,1.7μm)by gradient elution,using water and methanol as the mobile phase.The target compounds were analyzed with electrospray ion source in negtive ion mode under multi reaction monitoring(MRM)mode,quantified by isotope(tetrahydrocannabinol-D3,THC-D3)internal standard method.The results showed that the standard curves were good linear relationship in the range of 1.0-20 ng/mL for CBD and THC,with the linear correlation coefficients greater than 0.995.The detection limits of the method(S/N=3)were 0.5,0.2μg/kg,for CBD and THC,respectively,and the spiked recoveries of six kinds of matrix samples were between 81.3%and 109.5%with RSD of 1.7%-11.4%.The method is simple,rapid,high sensitive and suitable for the determination of cannabidiol and tetrahydrocannabinol in cosmetics.

关 键 词：大麻二酚 四氢大麻酚 超高效液相色谱-串联质谱(UPLC-MS/MS) 化妆品 同位素稀释 

分 类 号：O657.63[理学—分析化学]