黄瓜内果皮汁液对种子萌发的影响  

Effects of Cucumber Endocarp Juice on Seed Germination

作　　者：杨艳 莫雨杏 周祎 陈惠明[3] 肖浪涛[1] 王若仲[1] YANG Yan;MO Yu-xing;ZHOU Yi;CHEN Hui-ming;XIAO Lang-tao;WANG Ruo-zhong(Hunan Provincial Key Laboratory of Phytohormones and Growth Development,College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128;Life Science and Technology School,Lingnan Normal University,Zhanjiang 524048;Vegetable Research Institute,Hunan Academy of Agricultural Science,Changsha 410125)

机构地区：[1]湖南农业大学生物科学技术学院植物激素与生长发育湖南省重点实验室,长沙410128 [2]岭南师范学院生命科学与技术学院,湛江524048 [3]湖南省农业科学院蔬菜研究所,长沙410125

出　　处：《生物技术通报》2023年第12期158-168,共11页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31871714);湖南省教育厅项目(20B306)。

摘　　要：明确黄瓜内果皮汁液在种子萌发中的作用、化学成分及机制,为基于黄瓜来源的种子萌发抑制剂研发提供一定的理论依据。以易胎萌黄瓜(C-L)种子为供试材料,研究难胎萌黄瓜(M-H)内果皮汁液(质量分数为100%、50%)及其不同提取液(质量分数为3%)对C-L种子萌发的影响,并分析汁液化学成分。针对质量分数为100%的M-H汁液和蒸馏水(对照)处理的种子生理生化指标动态变化进行分析。质量分数为100%、50%的M-H黄瓜内果皮汁液对C-L种子萌发均表现出显著的抑制作用,20 h抑制率分别为100%和73%。乙醇相、正丁醇相在种子整个萌发过程中均发挥抑制作用,与对照相比,26 h其抑制率分别提高12.22%和47.77%。在汁液中鉴定到影响种子萌发的化合物:肉豆蔻酸、棕榈酸、柠檬酸和组氨酸。质量分数为100%汁液处理的种子可溶性糖含量变化不大。植物激素ABA含量一直高于对照,ABA/GA30-20 h显著高于对照。ABA合成酶基因CsNCED110-15 h表达量增加,CsNCED215-25 h表达量显著增加;ABA信号转导基因CsPYL2、CsPP2C2、CsSnRK2和代谢基因CsCYP707A110 h后表达量均显著增加,20 h时表达量均最高。黄瓜内果皮汁液中的肉豆蔻酸、棕榈酸、柠檬酸、组氨酸可能通过促使ABA合成基因CsNCED2及信号转导基因CsPYL2、CsPP2C2、CsSnRK2表达量增加,从而使得ABA含量提高,同时阻碍可溶性糖代谢,进而有效抑制种子萌发。The roles,chemical composition and mechanisms of endocarp juice on seed germination were studied,which may provide a theoretical basis for the research of cucumber seed germination inhibitors from cucumber source.Viviparous cucumber(C-L)seed was used as materials and cultivated by not viviparous(M-H)endocarp juice(mass fractions was 100%and 50%)and its various extracts(mass fractions was 3%).The chemical composition of juice was analyzed.During seed germination,the physiological and biochemical factors were analyzed when the culture medium was a mass fraction of 100%juice and distilled water(control).Cucumber endocarp juice with a mass fraction of 100%and 50%effectively inhibited cucumber seed germination,with inhibition rates of 100%and 73%,respectively,at 20 h.Throughout the process,the EtOH and n-BuOH layers inhibited the seed germination,at 26 h,the inhibition rates were 12.22%and 47.77%higher than those of the control,respectively.Myristic acid,palmitic acid,citric acid,and histidine were identified in the juice that affected seed germination.The soluble sugar content of the seeds was insignificant change after the 100%juice treatment process.From 0 to 20 h,ABA content was always higher than that of the control,and ABA/GA3 was significantly higher than that of the control.The expressions of the ABA synthase genes CsNCED1 and CsNCED2 rose from 10 to 15 h and from 15 to 25 h,respectively.After 10 h,the expressions of ABA signal transduction genes CsPYL2,CsPP2C2,CsSnRK2,and catabolic gene CsCYP707A1 increased significantly,with the highest expression of four genes detected at 20 h.Cucumber endocarp juice contains myristic acid,palmitic acid,citric acid,and histidine,which may decrease seed germination by increasing the expressions of ABA synthesizing CsNCED2 and signal transduction genes CsPYL2,CsPP2C2,and CsSnRK2,as well as inhibiting soluble sugar metabolism.

关 键 词：黄瓜 内果皮汁液 种子萌发 化学成分 植物激素 ABA 

分 类 号：S642.2[农业科学—蔬菜学]