生防细菌HK11-9对黄瓜棒孢叶斑病的防病能力及其鉴定  

Control Ability and Identification of Biocontrol Agent HK11-9 Against Corynespora Leaf Spot of Cucumber

作　　者：张林林 沈虎生 杨冰 何梦菡 朴凤植[2] 申顺善[1] ZHANG Lin-lin;SHEN Hu-sheng;YANG Bing;HE Meng-han;PIAO Feng-zhi;SHEN Shun-shan(College of Plant Protection,Henan Agricultural University,Zhengzhou 450002;College of Horticulture,Henan Agricultural University,Zhengzhou 450002)

机构地区：[1]河南农业大学植物保护学院,郑州450002 [2]河南农业大学园艺学院,郑州450002

出　　处：《生物技术通报》2023年第12期209-218,共10页Biotechnology Bulletin

基　　金：河南省重点科技攻关资助项目(202102110218);国家重点研发计划项目(2017YFD0201100)。

摘　　要：为探讨黄瓜棒孢叶斑病生防细菌HK11-9的防病能力,通过皿内试验测定HK11-9对黄瓜棒孢叶斑病菌多主棒孢霉的抑菌活性、通过离体和活体接种试验评价其对黄瓜棒孢叶斑病的防治效果,进行盆栽试验分析HK11-9在黄瓜叶部的定殖能力及其对黄瓜叶部防御酶活性的影响,同时,测定HK11-9的生物学功能并对其进行鉴定。结果显示,HK11-9显著抑制多主棒孢霉的菌丝生长和孢子萌发;在离体接种和活体接种条件下,HK11-9均对黄瓜棒孢叶斑病具有明显的防治效果,其防治效果分别达到62.80%和67.73%;同时,HK11-9在黄瓜叶部具有稳定的定殖能力,在黄瓜叶部能保持10^(4) CFU/g的定殖密度,能够显著提高黄瓜叶部PAL、POD和PPO活性。HK11-9菌株具有分解蛋白、分解纤维素、分解淀粉和产嗜铁素作用,对多种植物病原真菌具有抑菌活性;并根据形态特征、生理生化特性和16S rRNA序列同源性分析,将HK11-9菌株鉴定为多黏类芽孢杆菌(Paenibacillus polymyxa)。综上所述,生防细菌HK11-9防治黄瓜棒孢叶斑病具有极大的潜力。In order to explore the biocontrol ability of biocontrol agent HK11-9 against Corynespora leaf spot of cucumber,the antifungal activity of HK11-9 against Corynespora cassiicola were detected by in petri dishes experiments,the control effect of HK11-9 against Corynespora leaf spot of cucumber were detected by in vitro and in vivo inoculation experiments,the colonization ability of HK11-9 on cucumber leaves and its effect on the activities of defense enzymes were conducted by pot experiment,at the same time,the biological functions of HK11-9 was determined.The results showed that,HK11-9 significantly inhibited the mycelia growth and conidia germination of Corynespora cassiicola in the petri dishes experiment,HK11-9 presented significant control effect against Corynespora leaf spot of cucumber at the in vitro and in vivo inoculation experiment,the control efficiencies were 62.80%and 67.73%respectively.HK11-9 colonized stably in cucumber leaves,maintained a density of 10^(4) CFU/g in cucumber leaves,and HK11-9 significantly improved the activities of defense enzymes in cucumber leaves.In addition,HK11-9 decomposed protein,cellulose and starch,produced siderophore,and HK11-9 demonstrated antifungal activity against a variety plant pathogenic fungi.Based on the morphological,physiological characteristics and 16S rRNA sequence analysis,the strain HK11-9 was identified as Paenibacillus polymyxa.In summary,the biocontrol agent HK11-9 has great potential to control Corynespora leaf spot of cucumber.

关 键 词：黄瓜棒孢叶斑病 多黏类芽孢杆菌HK11-9 定殖量 防御酶活性 鉴定 生物防治 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S436.421[农业科学—植物保护]