Atteva charopis的比较线粒体基因组研究及系统发育分析  

Comparative Mitochondrial Genome and Phylogenetic Analysis of Atteva charopis Turner,1903

作　　者：林兴雨 宋南[1] LIN Xing-yu;SONG Nan(College of Plant Protection,Henan Agricultural University,Zhengzhou 450046)

机构地区：[1]河南农业大学植物保护学院,郑州450046

出　　处：《生物技术通报》2023年第12期300-310,共11页Biotechnology Bulletin

基　　金：国家自然科学基金项目(U1904104)。

摘　　要：线粒体基因组有助于对巢蛾总科的分子进化和系统发育的理解。本研究测序、分析了Atteva charopis Turner,1903的线粒体全基因组,并基于线粒体基因组的核苷酸和氨基酸数据重建了巢蛾总科的系统发育关系。在系统发育分析中,内群取样包含巢蛾总科的10种昆虫,外群选择2个谷蛾总科Tineoidea(Phyllocnistis citrella和Caloptilia theivora)昆虫;分别利用最大似然法和贝叶斯法重建巢蛾总科的系统发育关系。Atteva charopis的线粒体基因组全长为15163 bp(GenBank序列号:OQ130005),包含37个基因(13个蛋白质编码基因、2个核糖体RNA基因和22个转运RNA基因)和一段非编码控制区。与假定昆虫的trn基因(trnI-trnQ-trnM)相比,该线粒体基因出现了trn基因重排(trnI-trnM-trnQ)。两种不同的系统发育分析方法构建的系统发育关系树显示,潜叶蛾科、Praydidae和斑巢蛾科为单系群,而菜蛾科为非单系群,斑巢蛾科和Praydidae亲缘关系较近,Atteva charopis与Atteva aurea形成姐妹群。本研究结果可为深入研究巢蛾总科的系统发育提供线粒体基因组数据。Mitochondrial genomes(mitogenomes)advance our understanding of molecular evolution and phylogenetic relationships in Yponomeutoidea.In this study,we sequenced and analyzed the mitochondrial genome of Atteva charopis Turner,1903.We reconstructed the phylogenetic relationships of Yponomeutoidea based on the nucleotide and amino acid data of the mitochondrial genome.In phylogenetic analyses,we selected 10 exemplars of Yponomeutoidea as ingroups,and two species of Tineoidea(Phyllocnistis citrella and Caloptilia theivora)as outgroups.The phylogenetic analyses were conducted using Maximum likelihood and Bayesian inference methods.The mitochondrial genome of Atteva charopis was a circular molecule of 15163 bp in length(GenBank accession No.OQ130005),containing 13 protein-coding genes,two ribosomal RNA genes,22 transfer RNA genes and a non-coding control region.Compared to the putative insect trn gene(trnI-trnQ-trnM),there has been a trn gene rearrangement(trnI-trnM-trnQ)in this mitochondrial genome.Both phylogenetic inference methods produced a similar tree topological structure:Family Lyonetiidae,Praydidae and Attevidae were the monophyletic groups.However,the family Plutellidae was the non-monophyletic group.The family Attevidae was closely related to Praydidae.In addition,A.charopis was the sister to A.aurea.Our results provide a mitochondrial genome data for the intensive study of the phylogeny of Yponomeutoidea.

关 键 词：鳞翅目 巢蛾总科 比较线粒体基因组 系统发育 

分 类 号：Q963[生物学—昆虫学]