β-甘露聚糖酶对鹌鹑生产性能、血浆生化指标及肠道微生物区系的影响  

Effects ofβ-Mannanase on Performance,Plasma Biochemical Indices and Intestinal Microflora of Egg Quail

作　　者：霍文颖 李梦云 车龙 王彩玲 王志祥[2] HUO Wenying;LI Mengyun;CHE Long;WANG Cailing;WANG Zhixiang(College of Animal Science and Technology,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;College of Animal Science and Technology,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南牧业经济学院动物科技学院,郑州450046 [2]河南农业大学动物科技学院,郑州450046

出　　处：《动物营养学报》2023年第12期7839-7851,共13页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：河南牧业经济学院博士科研启动资金项目(2019HNUAHEDF039)。

摘　　要：本试验旨在研究β-甘露聚糖酶对产蛋高峰期鹌鹑生产性能、养分表观代谢率、蛋品质、血浆生化指标、肠道结构和肠道微生物区系的影响。选取240只健康、体重接近的产蛋高峰期(80日龄)鹌鹑,随机分为2组,每组6个重复,每个重复20只。对照组饲喂基础饲粮,试验组在基础饲粮中添加200 mg/kg的β-甘露聚糖酶。预试期30 d,正试期120 d。结果显示:与对照组相比,添加β-甘露聚糖酶极显著提高了十二指肠和回肠的绒毛高度和绒隐比、Chao1指数、ACE指数、α-变形菌纲(Alphaproteobacteria)和梭菌纲(Clostridia)的相对丰度(P<0.01),极显著改善了蛋形指数(P<0.01),显著提高了鹌鹑的平均蛋重、能量和粗蛋白质的表观代谢率、血浆总蛋白(TP)和白蛋白(ALB)的含量以及丹丝毒菌纲(Erysipelotrichia)的相对丰度(P<0.05),显著改善了蛋壳强度、蛋黄颜色和哈氏单位(P<0.05),此外,极显著降低了死淘率、十二指肠的隐窝深度和Negativicutes的相对丰度(P<0.01),显著降低了料蛋比、血浆肌酐(CRE)含量、谷丙转氨酶(ALT)和谷草转氨酶(AST)的活性、白细胞介素-1(IL-1)的含量(P<0.05)。综上所述,在产蛋高峰期鹌鹑饲粮中添加200 mg/kg的β-甘露聚糖酶可改善盲肠微生物区系的组成,提高鹌鹑的生产性能、养分表观代谢率、蛋品质、免疫力和肠道的健康。This experiment investigated the effects ofβ-mannanase on performance,nutrient apparent metabolic rates,egg quality,plasma biochemical indices,intestinal structure,and intestinal microflora of quail during the peak laying period.A total of 240 healthy quail(80 days of age)with similar body weight at the peak of egg production were randomly divided into two groups:six replicates per group and 20 quail per replicate.Quail in the control group were fed a basal diet,and those in the experimental group were fed the basal diet supplemented with 200 mg/kgβ-mannanase.The pre-test period was 30 days,and the test period was 120 days.The results showed that compared with the control group,the addition ofβ-mannanase significantly increased the villus height and ratio of villus height to crypt depth,Chao1 index,ACE index,and the relative abundance of Alphaproteobacteria and Clostridia in duodenum and ileum(P<0.01),significantly improved egg shape index(P<0.01),significantly increased the average egg weight,apparent metabolic rates of energy and crude protein,the contents of total protein(TP)and albumin(ALB)in plasma and the relative abundance of Erysipelotrichia(P<0.05),significantly improved egg shell strength,egg yolk color and Haugh unit(P<0.05).In addition,the death rate,duodenal crypt depth and relative abundance of Negativicutes significantly reduced(P<0.01),and the ratio of feed to egg,the content of creatinine(CRE),the activities of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)and the content of interleukin-1(IL-1)significantly decreased(P<0.05).In conclusion,dietary supplementation of 200 mg/kgβ-mannanase can improve the composition of cecal microflora,performance,nutrient apparent metabolic rate,egg quality,immunity,and intestinal health of quail during the peak laying period.

关 键 词：Β-甘露聚糖酶 鹌鹑 生产性能 血浆生化指标 肠道微生物区系 

分 类 号：S839[农业科学—畜牧学]