沙棘提取物促进大鼠肛周脓肿术后创面愈合效果及对p38/MK2通路的影响  

Effect of sea buckthorn extract on promoting wound healing after perianal abscess in rats and its effect on the p38/MK2 pathway

作　　者：童偲辰 TONG Caichen(General Surgery Department,Beijing Heping Li Hospital,Beijing 100000,China)

机构地区：[1]北京市和平里医院普外科,北京100000

出　　处：《安徽医药》2024年第1期26-30,I0003,共6页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨沙棘提取物对大鼠肛周脓肿术后创面愈合及p38/丝裂原活化蛋白激酶2(MK2)通路的影响。方法2021年6—12月,选取SD大鼠60只,采用随机数表法分成假手术组、模型组、阳性对照组(左氧氟沙星,63 mg/kg)、沙棘提取物低(5 g/kg)、中(10 g/kg)、高(20 g/kg)剂量组,每组10只。构建肛周脓肿术后大鼠模型,测量大鼠术后0 d和观察期(7 d和14 d)的创面大小,计算创面愈合率;采用ELISA法检测大鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β和IL-6水平;观察大鼠创面组织病理改变;应用实时荧光定量PCR(RT-qPCR)法检测大鼠创面组织p38、MK2mRNA,蛋白质印迹法检测大鼠创面组织p38、MK2蛋白。结果模型组大鼠创面组织表皮厚度最薄,细胞边界模糊,细胞排列紊乱,阳性对照组和沙棘提取物各剂量组表皮厚度明显增加,且随着沙棘提取物剂量的增加表皮厚度逐渐增厚。与假手术组比较,创面组织p38、MK2 mRNA(1.00±0.02比1.82±0.12,1.00±0.03比1.70±0.14)和创面组织p38、MK2蛋白(0.61±0.07比0.89±0.08,0.63±0.07比0.92±0.10)表达水平显著升高(P<0.05)。与模型组比较,阳性对照组和沙棘提取物各剂量组大鼠创面愈合率显著升高,血清TNF-α、IL-1β和IL-6水平、创面组织p38、MK2 mRNA(1.82±0.12比0.69±0.07、1.35±0.10、0.96±0.09、0.75±0.09,1.70±0.14比0.61±0.08、1.30±0.11、0.98±0.06、0.66±0.07)以及创面组织p38、MK2蛋白(0.89±0.08比0.29±0.03、0.75±0.09、0.56±0.08、0.32±0.04,0.92±0.10比0.33±0.04、0.80±0.09、0.59±0.07、0.36±0.06)表达水平显著降低(P<0.05)。结论沙棘提取物可有效提高大鼠肛周脓肿术后创面的愈合效果,其机制可能与降低大鼠血清炎性因子,并抑制p38/MK2通路相关蛋白表达有关。Objective To investigate the effect of sea buckthorn extract on wound healing and the p38/mitogen-activated protein ki⁃nase 2(MK2)pathway after perianal abscess surgery in rats.Methods From June to December 2021,60 SD rats were selected and randomly divided into a sham operation group,a model group,a positive control group(levofloxacin,63 mg/kg),and a sea buckthorn ex⁃tract low(5 g/kg),medium(10 g/kg),and high(20 g/kg)dose group by the random number table method,with 10 rats in each group.A rat model of postoperative perianal abscess was constructed,and the wound size at 0 d and the observation period(7 d and 14 d)after surgery was measured to calculate the wound healing rate.ELISA was used to measure the levels of tumor necrosis factor-α(TNF-α),in⁃terleukin-1β(IL-1β),and interleukin-6(IL-6)in the serum of rats.Pathological changes in rat wound tissue were observed.Real-time fluorescence quantitative PCR(RT-qPCR)was used to detect p38 and MK2 mRNA in rat wound tissue,and Western blotting method was used to detect p38 and MK2 protein in rat wound tissue.Results In the model group,the epidermal thickness of the wound tissue in the model group was the thinnest,with blurred cell boundaries and disordered cell arrangement.The epidermal thickness of the posi⁃tive control group and sea buckthorn extract dosage group increased significantly,and the epidermal thickness gradually thickened with increasing sea buckthorn extract dosage.Compared with the sham-operated group,the expression levels of p38 and MK2 in wound tis⁃sue(1.00±0.02 vs.1.82±0.12,1.00±0.03 vs.1.70±0.14)and the prote in levels of p38 and MK2 in wound tissue(0.61±0.07 vs.0.89±0.08,0.63±0.07 vs.0.92±0.10)were significantly increased(P<0.05).Compared with the model group,the wound healing rate of rats in the positive control group and each dose group of sea buckthorn extract was significantly increased,and the TNF-α,IL-1β,IL-6 lev⁃els in serum,mRNA levels of p38,and MK2 in wound tissue(1.82±0.12 vs.0.69±0.07,1.35±0.10,0.96±0.09,0.

关 键 词：肛周脓肿 沙棘提取物 p38/丝裂原活化蛋白激酶2通路 大鼠 Sprague-Dawley 

分 类 号：R285.5[医药卫生—中药学]