子痫前期病人长链非编码RNA H19和长链非编码RNA HAND2-AS1表达水平与胰岛素抵抗的相关性研究  

Correlation between the expression levels of LncRNA H19 and LncRNA HAND2-AS1 and insulin resistance in patients with preeclampsia

作　　者：蒋天从 刘志明 谷孝月 郭婉茹[1] 石冲[1] 戚桂杰[1] JIANG Tiancong;LIU Zhiming;GU Xiaoyue;GUO Wanru;SHI Chong;QI Guijie(Antenatal Diagnosis Department of Tangshan Maternal and Child Health Hospital,Tangshan KeyLaboratory of Birth Defect Screening and Diagnosis,Tangshan,Hebei 063000,China)

机构地区：[1]唐山市妇幼保健院产前诊断科、唐山市出生缺陷筛查与诊断重点实验室,河北唐山063000

出　　处：《安徽医药》2024年第1期49-53,共5页Anhui Medical and Pharmaceutical Journal

基　　金：2022年度河北省医学科学研究项目(20221770)。

摘　　要：目的检测子痫前期病人胎盘组织、血清长链非编码RNA H19(LncRNA H19)与长链非编码RNAHAND2-AS1(Ln⁃cRNA HAND2-AS1)表达水平,并分析其与病人胰岛素抵抗(IR)的相关性。方法将2021年1—12月在唐山市妇幼保健院建卡的子痫前期孕妇105例作为子痫前期组,根据子痫前期孕妇严重程度分为轻度子痫前期组与重度子痫前期组,另选取同期孕周、年龄相符的健康孕妇97例作为对照组,收集所有孕妇身体质量指数(BMI)、孕周、收缩压、舒张压、空腹胰岛素(FINS)、空腹血糖(FBG)等一般资料,并计算稳态模型的IR指数(HOMA-IR);实时荧光定量PCR(qRT-PCR)法测定孕妇血清、胎盘组织中LncRNA H19,LncRNA HAND2-AS1水平;比较对照组与子痫前期组、轻度子痫前期组与重度子痫前期组间血清、胎盘组织LncRNA H19、LncRNA HAND2-AS1水平;Pearson法分析子痫前期孕妇血清、胎盘组织LncRNA H19、LncRNA HAND2-AS1与HOMA-IR的相关性。结果子痫前期组病人收缩压、舒张压高于对照组(P<0.05),且重度子痫前期病人收缩压、舒张压高于轻度子痫前期病人,孕周低于轻度子痫前期病人(P<0.05);与对照组相比,子痫前期组孕妇24 h尿蛋白、FBG[(5.84±0.97)mmol/L比(4.22±0.70)mmol/L]、FINS[(13.37±2.23)mU/L比(7.52±1.25)mU/L]、HOMI-IR水平(3.47±0.57比1.41±0.23)升高(P<0.05),且重度子痫前期孕妇24 h尿蛋白、FBG[(6.90±1.15)mmol/L比(5.24±0.85)mmol/L]、FINS[(13.97±2.32)mU/L比(13.05±2.17)mU/L]、HOMI-IR水平(4.27±0.71比3.03±0.50)高于轻度子痫前期孕妇(P<0.05);与对照组相比,子痫前期组孕妇血清、胎盘组织Ln⁃cRNA H19(2.52±1.42比1.01±0.15,3.75±0.62比1.02±0.17)与LncRNA HAND2-AS1水平(1.98±0.33比1.01±0.15,2.87±0.47比1.02±0.17)升高(P<0.05),且重度子痫前期孕妇血清、胎盘组织LncRNA H19(2.84±0.47比2.34±0.39,4.28±0.71比3.45±0.57)与LncRNA HAND2-AS1水平(2.59±0.43比1.63±0.27,3.56±0.59比2.49±0.41)高于轻度子痫前期孕妇(P<0.05);子痫Objective To detect the expression levels of long non-coding RNA H19(LncRNA H19)and long non-coding RNA HAND2-AS1(LncRNA HAND2-AS1)in placental tissue and serum of patients with preeclampsia,and to analyze their correlation with insulin resistance(IR).Methods One hundred and five pregnant women with preeclampsia who established cards in Tangshan Mater⁃nal and Child Health Hospital from January 2021 to December 2021 were taken as the preeclampsia group.According to the severity of preeclampsia,pregnant women were assigned into mild preeclampsia group and severe preeclampsia group.Another 97 healthy preg⁃nant women with the same gestational age and age were taken included in the control group.General information such as body mass in⁃dex(BMI),gestational age,systolic blood pressure,diastolic blood pressure,fasting insulin(FINS),and fasting blood glucose(FBG)of all pregnant women were collected,and the IR index of the steady state model(HOMA-IR)was calculated;Real-time quantitative re⁃verse transcription PCR(qRT-PCR)method was used to determine the levels of LncRNA H19 and LncRNA HAND2-AS1 in maternal Anhui Medical and Pharmaceutical Journal 2024 Jan,28(1)serum and placental tissue;the levels of LncRNA H19 and LncRNA HAND2-AS1 in serum and placental tissue were compared be⁃tween the control group,the preeclampsia group,the mild preeclampsia group and the severe preeclampsia group;Pearson's method was used to analyze the correlation between serum and placental tissue LncRNA H19,LncRNA HAND2-AS1 and HOMA-IR of pre⁃eclampsia pregnant women.Results The systolic blood pressure and diastolic blood pressure in the preeclampsia group were higher than those in the control group(P<0.05),and the systolic blood pressure and diastolic blood pressure in the patients with severe pre⁃eclampsia were higher than those in the patients with mild preeclampsia,and the gestational age was lower than that in the patients with mild preeclampsia(P<0.05);compared with the control group,the levels of 24-hour urine protein,FBG[(5.8

关 键 词：先兆子痫 RNA 长链非编码 LncRNA H19 LncRNA HAND2-AS1 胰岛素抵抗 

分 类 号：R714.244[医药卫生—妇产科学]