地衣芽孢杆菌产芽孢培养基和培养条件的优化  

Optimization on sporulation medium and culture conditions of Bacillus licheniformis

作　　者：董雪 刘晓露 李丽蓓[1] 刘妩妍 雷浩 樊霞[1] DONG Xue;LIU Xiaolu;LI Libei;LIU Wuyan;LEI Hao;FAN Xia(Institute of Quality Standard and Testing Technology for Agro-Products,Chinese Academy of Agricultural Science,Beijing 100081,China;Shaanxi Qinyun Agricultural Products Inspection and Testing Co.,Ltd.,Weinan,Shaanxi Province 714000,China)

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所,北京海淀100081 [2]陕西秦云农产品检验检测股份有限公司,陕西渭南714000

出　　处：《中国饲料》2024年第1期36-43,50,共9页China Feed

基　　金：饲用地衣芽孢杆菌检测用标准样品(S2022115);基于数字信息技术的中欧食品安全过程控制体系的建立与示范(2019YFE0103800)。

摘　　要：本研究以地衣芽孢杆菌CICC 20514为对象,芽孢数和芽孢率为目标参数,采用单因素、正交试验方法对培养基配方进行筛选,并在此配方基础上对培养条件进一步优化。结果表明:最终产芽孢发酵培养基的最佳组合为20 g/L果糖、20 g/L豆粕、5 g/L K2HPO_(4)、7 g/L NaCl、0.8 g/L CaCO_(3)和0.8 g/L MgSO_(4);产芽孢最佳培养条件为37℃,220 r/min,接种量5%,装样量20%,初始pH 6.5~7.5,发酵培养时间48 h。优化后的地衣芽孢杆菌CICC 20514培养基芽孢数及芽孢率均具有良好的稳定性,发酵液芽孢数达5.8×10^(9)CFU/mL,比优化前提高了58倍。The medium formulation was screened by single-factor and orthogonal tests for Bacillus licheniformis CICC 20514 with the number and rate of spores as a screening condition,and the culture conditions were further optimized on the basis of this formulation.The results showed that the optimal combination of spore fermentation medium contains 20 g/L fructose,20 g/L soybean meal,5 g/L K2HPO_(4),7 g/L NaCl,0.8 g/L CaCO_(3) and 0.8 g/L MgSO_(4).The optimal culture conditions for spores production were 37℃,220 r/min,5%inoculum,20%sample loading,initial pH 6.5~7.5 and 48 h of fermentation.The optimized Bacillus licheniformis CICC 20514 medium had good stability in spore number and rate.The number of spores in the fermentation reached 5.8×10^(9) CFU/mL,which was 58 times higher than before optimization.

关 键 词：地衣芽孢杆菌 发酵培养基 培养条件 

分 类 号：S816.7[农业科学—饲料科学]