抗菌肽对大口黑鲈生长代谢的影响研究  

Effects of antimicrobial peptides on growth and metabolism of Micropterus salmoides

作　　者：宣雄智[1] 赵宇凡 朱文婷 范勇 赵永志 郭益红[1] 李文嘉 XUAN Xiongzhi;ZHAO Yufan;ZHU Wenting;FAN Yong;ZHAO Yongzhi;GUO Yihong;LI Wenjia(Suzhou Polytechnic Institute of Agriculture,Suzhou,Jiangsu Province 215008,China;Daxin County Agricultural Technology Extension Service Center Zhangjiagang,Jiangsu Province 215636,China;Guangdong Hainachuan Biotechnology Co.,Ltd,Foshan,Guangdong Province 528515,China;Institute of Animal Husbandry,Henan Academy of Agricultural Sciences,Zhengzhou,Henan Province 450002,China)

机构地区：[1]苏州农业职业技术学院,江苏苏州215008 [2]张家港市大新镇农技推广服务中心,江苏张家港215636 [3]广东海纳川生物科技有限公司,广东佛山528515 [4]河南省农业科学院畜牧研究所,河南郑州450002

出　　处：《中国饲料》2024年第1期119-124,共6页China Feed

基　　金：苏州农业职业技术学院博士提升计划科研启动基金项目(19BS1002);江苏现代农业(大宗鱼类)产业技术体系建设项目(JATS[2023]350);苏州市科技计划项目(SNG2023012);河南省重点研发与推广专项(科技攻关[232102110120]);河南省农业科学院自主创新项目(2023ZC054)。

摘　　要：为评估不同水平抗菌肽对大口黑鲈生长代谢的影响,试验以不含抗菌肽的基础饲料为对照组,在基础饲料中分别添加50 mg/kg(D1组),100 mg/kg(D2组),150 mg/kg(D3组),200 mg/kg(D4组)抗菌肽为试验组,在养殖水箱中饲喂初始平均体质量(12.49±0.2)g的大口黑鲈幼鱼,养殖8周后测定试验鱼的生长性能、饲料表观消化率、鱼体成分、营养代谢等相关指标。试验结果表明:(1)生长和体组成方面,各组试验鱼增重率均高于对照组但差异不显著(P>0.05)。与对照组相比,D4组干物质消化率显著升高6.58%(P<0.05);D3和D4组饲料转化率分别显著升高9.4%和10.8%(P<0.05);各组试验鱼全鱼粗蛋白质、粗脂肪和粗灰分均无显著差异(P>0.05),D3组脏体比比对照组显著升高9.4%(P<0.05);(2)消化吸收方面,与对照组相比,D2和D3组前肠钠钾ATP酶活性分别显著升高32.35%和39.04%(P<0.05),前肠碱性磷酸酶活性分别显著升高12.09%和10.05%(P<0.05),肝脏溶菌酶活性分别显著升高11.81%和10.9%(P<0.05),D3组前肠脂肪酶活性显著升高12.62%(P<0.05);(3)蛋白质代谢方面,与对照组相比,D3和D4组血清总蛋白含量分别显著升高19.53%和18.7%(P<0.05),D3和D4组蛋白质效率分别显著升高10.31%和10.82%(P<0.05),D4组肝脏AST活性显著升高22.49%(P<0.05);(4)脂肪代谢方面,D4组血清总胆固醇含量比对照组显著下降18.76%(P<0.05)。本研究表明,在大口黑鲈饲料中添加抗菌肽可以提高饲料转化率,促进蛋白质沉积,能在一定程度上提升鱼体免疫性能,抗菌肽添加量为150 mg/kg时效果较佳。The diets supplemented with 0 mg/kg,50 mg/kg(D1 group),100 mg/kg(D2 group),150 mg/kg(D3 group)or 200 mg/kg(D4 group)antibacterial peptide respectively were fed with the Juvenile Micropterus salmoides[(12.49±0.2)g]for 8 weeks to investigate the effects of antibacterial peptide on growth and metabolism performance of the Micropterus salmoides.The growth performance,apparent digestibility of feed,fish body composition,nutrient metabolism and other related indicators of the fish were tested.The results indicated that:(1)In terms of growth and body composition,the weight gain of the fish in each group was higher than that of the control group,but the difference was not significant(P>0.05),compared with the control group,the dry matter digestibility of D4 group was significantly increased by 6.58%(P<0.05);The feed conversion rates of D3 and D4 groups were increased significantly by 9.4%and 10.8%respectively(P<0.05);There was no significant difference in crude protein,crude fat and crude ash of the whole fish in each group(P>0.05);(2)In terms of digestion and absorption,compared with the control group,the activities of Na+,K+-ATPase in the anterior intestine of D2 and D3 groups were significantly increased by 32.35%and 39.04%respectively(P<0.05),the activities of alkaline phosphatase in the anterior intestine of D2 and D3 groups were significantly increased by 12.09%and 10.05%respectively(P<0.05),the activities of lysozyme in the liver of D2 and D3 groups were significantly increased by 11.81%and 10.9%respectively(P<0.05),and the activity of lipase in the anterior intestine of D3 group was significantly increased by 12.62%(P<0.05);(3)In terms of protein metabolism,compared with the control group,the total protein in serum of D3 and D4 groups were significantly increased by 19.53%and 18.7%respectively(P<0.05),the protein efficiencies of D3 and D4 groups were significantly increased by 10.31%and 10.82%respectively(P<0.05),and the AST activity in liver of D4 group was significantly increased by 22.49%(P<0.05);(4)In terms

关 键 词：抗菌肽 大口黑鲈 蛋白质代谢 脂肪代谢 

分 类 号：S816.7[农业科学—饲料科学]