基于水稻大粒染色体片段代换系Z29的鉴定及QTL定位  

Identification and Localization of QTL Based on a Rice Large Grain Chromosome Segment Substitution Line Z29

作　　者：刘金艳 张朔语 宗涵颖 陈文博 韦秘 吴如会 母建妍 张记超 凌英华[1] 张长伟[1] 何光华[1] 赵芳明[1] 张婷 LIU Jinyan;ZHANG Shuoyu;ZONG Hanying;CHEN Wenbo;WEI Mi;WU Ruhui;MU Jianyan;ZHANG Jichao;LING Yinghua;ZHANG Changwei;HE Guanghua;ZHAO Fangming;ZHANG Ting(College of Agronomy and Biotechnology,Southwest University/Engineering Research Center of South Upland Agriculture(Ministry of Education),Chongqing 400715,China)

机构地区：[1]西南大学农学与生物科技学院/南方山地农业教育部工程中心,重庆400715

出　　处：《西南大学学报（自然科学版）》2024年第2期13-23,共11页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市技术创新与应用发展专项(CSTB2022TIAD-KPX0018);中央高校基本科研业务费项目(SWU-KT22041);国家级大学生创新创业训练计划项目(202310635055).

摘　　要：千粒质量作为水稻产量的三要素之一,对产量的影响较大,其中千粒质量主要受水稻粒型的影响,因此挖掘新的粒型基因在生产中具有重要的意义.研究选育到1个以日本晴为受体亲本、自育优良籼稻恢复系R225为供体亲本的水稻染色体片段代换系(CSSL)Z29. Z29含有来自R225的10个代换片段,平均代换长度2.90 Mb. Z29粒长和粒宽均极显著增加,表现为大粒表型,且其籽粒变大是由颖壳细胞数量极显著增多、增大引起.利用日本晴与Z29杂交构建的次级F2群体进行QTL定位,共检测到8个粒型相关QTL.进一步利用MAS法在F3群体中选育出14个次级染色体片段代换系,包括4个单片段代换系、 5个双片段代换系、 2个三片段代换系和3个四片段代换系.结果可为目的粒型相关QTL克隆和分子机制解析奠定基础,为分子设计育种提供资源.As one of the three elements of rice yield,grain size has an important impact on yield.It is important to explore new genes for grain development.In this study,a rice chromosomal segment substitution line Z29 was selected from a cross with Nipponbare as the recipient and the self-bred indica rice restorer line R225 as the donor parent.Z29 contains 10 substitution segments from R225 with an average substitution length of 2.90 Mb.Both grain length and width of Z29 were increased significantly,showing a large grain phenotype,and its grain enlargement was caused by the highly significant increase in the number and size of glume cells.A total of eight grain shape-related QTLs were detected in the secondary F2 population constructed by crossing Z29 with Nipponbare.Fourteen secondary chromosome segment substitution lines were selected from F3 population using MAS method,including four single segment substitution lines,five double segment substitution lines,two triple segment substitution lines and three quadruple segment substitution lines.These results lay the foundation for cloning grain shape-related QTLs and elucidation of the molecular mechanism,and provide resources for molecular design breeding.

关 键 词：水稻 染色体片段代换系 粒型 QTL定位 产量 

分 类 号：S511[农业科学—作物学]