水稻早衰突变体psl4(t)的表型鉴定及基因定位  

Phenotypic Characterization and Gene Mapping of a Presenescing Leaf Mutant psl4(t)in Rice(Oryza sativa L.)

作　　者：龚晓平 何焕然 莫春红 李加胜 陈锐 张致力 孙小红 勾治琴 罗挺 GONG Xiaoping;HE Huanran;MO Chunhong;LI Jiasheng;CHEN Rui;ZHANG Zhili;SUN Xiaohong;GOU Zhiqin;LUO Ting(Southeast Chongqing Academy of Agricultural Sciences,Fuling Chongqing 408000,China)

机构地区：[1]重庆市渝东南农业科学院,重庆涪陵408000

出　　处：《西南大学学报（自然科学版）》2024年第2期44-51,共8页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市技术创新与应用发展专项(CSTB2022TIAD-KPX0018,CSTB2022TIAD-KPX0009);“重庆英才计划”创新创业示范团队项目(cqyc201903226);重庆市农业产业技术体系“水稻创新团队”项目(COMAITS202301);浙江省科技厅对口帮扶项目(2021C04025)。

摘　　要：水稻叶片早衰严重影响水稻的产量与品质,挖掘水稻叶片早衰相关基因并解析其分子机制,对提高水稻产量具有重要意义.从涪恢9802和LR杂交后代中筛选到叶早衰突变体psl4(t),该突变体6叶期前叶片呈正常绿色,从7叶期至剑叶(倒1叶)期每张叶片均是从叶尖至叶中部逐渐衰老,叶片的叶绿体发育受阻其体积变小、光合色素质量分数减少,叶片提前衰老.农艺性状分析结果表明:与野生型相比,突变体psl4(t)的穗长、有效分蘖数和籽粒宽变化无统计学意义,而株高、每穗粒数、结实率及千粒质量显著降低.遗传分析结果发现:突变体psl4(t)的早衰性状受单隐性核基因控制,利用分子标记将目标基因定位于第4染色体长臂端两个SSR标记(RM17004和RM17006)之间38.5 kb的范围内.研究为psl4(t)基因的克隆及功能解析、早衰分子机制研究奠定基础.Early leaf senescence greatly affects grain yield and quality in rice.It is of great significance to mine genes related to rice leaf premature senescence and analyze their molecular mechanisms to improve rice yield.A presenescing leaf mutant psl4(t)was obtained from the hybrid offspring of Fuhui9802 and LR in this study.The psl4(t)mutant exhibited a normal phenotype up to the L6 stage.Nevertheless,from seventh leaf to flag leaf,each leaf gradually senesced from leaf tip to middle of leaf.The chloroplast development of leaves was inhibited,the size of chloroplasts became smaller,the content of photosynthetic pigments decreased,which leads to the advanced leaf senescence.The results of agronomic trait analysis showed that compared with the wild type,the psl4(t)mutant had no significant changes in panicle length,effective tiller number and seed width,while plant height,grain number per panicle,seed setting rate and thousand kernel weight significantly reduced.Genetic analysis revealed that the premature senescence trait of psl4(t)mutant was controlled by a single recessive gene.The target gene was mapped in a 38.5 kb region on chromosome 4 between marker RM17004 and RM17006 through gene mapping.This study provides a basis for psl4(t)gene cloning and functional analysis,and molecular mechanism of premature leaf senescence.

关 键 词：水稻 早衰突变体psl4(t) 遗传分析 基因定位 

分 类 号：S511[农业科学—作物学]