亲吻素在青藏高原牦牛睾丸组织中的表达及定位研究  

Histology and kisspeptin expression in the testis of the Tibetan Plateau yak

作　　者：丁艳平[1] 赵越 张晋平[1] 马佳乐 DING Yan-ping;ZHAO Yue;ZHANG Jin-ping;MA Jia-le(College of Life Science,Northwest Normal University,Lanzhou 730070,Gansu,China)

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2024年第1期70-75,共6页Journal of Northwest Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(31760271)。

摘　　要：亲吻素(Kisspeptin)通过下丘脑-垂体-性腺轴对动物繁殖起重要的调控作用.为了探讨亲吻素在牦牛精子发生中的生理功能及其对高原环境的适应机制,文中对比研究了牦牛和黄牛睾丸的组织学特征及其亲吻素的表达情况.结果表明,与黄牛相比,牦牛的曲细精管不饱满,除精原细胞外,牦牛初级精母细胞、次级精母细胞、早期/晚期精子细胞以及精子的数量都显著少于黄牛(P<0.01).免疫组织化学结果显示,在牦牛的精原细胞、初级精母细胞、次级精母细胞、早期精细胞及基质细胞中都可见Kisspeptin免疫阳性产物的表达,这与黄牛表达位置一致,但是牦牛睾丸中阳性产物光密度值(IOD)却显著高于黄牛;牦牛睾丸组织中Kisspeptin蛋白的表达显著高于黄牛(P<0.01).该结果暗示Kisspeptin可能作为负调控因子,参与维持牦牛精子发生和精子成熟过程中的正常组织结构及其微环境生理状态.Kisspeptin plays an important role in animal reproduction through the hypothalamic-pituitary-gonadal axis.In order to investigate the physiological function of kisspeptin in yak spermatogenesis and its adaptation mechanism to plateau environment,the histological characteristics and expression of kisspeptin in testis of yak and cattle were compared.The results showed that compared with cattle,the number of primary spermatocyte,secondary spermatocyte,early/late spermatocyte and spermatocyte in yak are significantly lower than that in cattle(P<0.01).Immunohistochemical results showed that kisspeptin immunopositive products are expressed in spermatogonium,primary spermatocyte,secondary spermatocyte,early spermatocyte and stromal cell of yak,which are consistent with the expression position of cattle,but the IOD value of positive substances in testis of yak is significantly higher than that of cattle.The expression of kisspeptin protein in the testicular tissue of yaks is significantly higher than that of cattle(P<0.01).The results showed that kisspeptin,as a negative regulator,is involved in maintaining the normal tissue structure and microenvironmental physiological state of yak spermatogenesis and sperm maturation.

关 键 词：牦牛 睾丸 亲吻素 Kiss-1阳性产物 精子发生 

分 类 号：S826[农业科学—畜牧学]