一株多药耐药Comamonas kerstersii细菌的基因组分析  

Genomic Insights of A Multi-Drug Resistant Comamonas kerstersii Isolate

作　　者：王慧[1] 明德松[2] 王明席[1] WANG Hui;MING Desong;WANG Mingxi(School of Medicine,Huaqiao University,Xiamen 361021,China;Quanzhou First Hospital Affiliated to Fujian Medical University,Quanzhou 362000,China)

机构地区：[1]华侨大学医学院,福建厦门361021 [2]福建医科大学附属泉州第一医院,福建泉州362000

出　　处：《华侨大学学报（自然科学版）》2024年第1期35-46,共12页Journal of Huaqiao University(Natural Science)

基　　金：福建省厦门南方海洋研究中心科研资金资助项目(14GYY008NF08);方树福堂基金会和方润华基金会资助项目(14X30127)。

摘　　要：从1名尿路感染患者中分离出了1株多药耐药的Comamonas kerstersii(C.kerstersii)菌株121606,对其进行了抗微生物药敏试验(AST)和全基因组测序;然后将其与7个具有代表性的Comamonas菌株和Acidovorax菌种进行基因组比较分析,包括使用OrthoANI分析平均核苷酸同一性(ANI),以及通过snpTree网络服务器进行单核苷酸多态性(SNP)分析。最后,使用RAST服务器进行基因组序列,使用OrthoVenn软件对同源簇进行功能注释,通过CARD数据库对抗生素耐药基因(ARGs)进行预测,并利用CRISPR识别工具预测CRISPR,以及利用PHAST软件预测前噬菌体。结果表明:C.kerstersii 121606是一种多药耐药细菌,其遗传成分与其他7个Comamonas和Acidovorax菌种相似;其基因组中存在的ARGs有助于解释其多药耐药机制。这些发现为研究新型抗生素来控制多药耐药C.kerstersii感染提供了有价值的见解。A multi-drug resistant C.kerstersii 121606 was isolated from a patient with a urinary tract infection,and the antimicrobial susceptibility testing(AST)and whole genome sequencing were performed,then comparative genomic analyses were conducted with seven representative Comamonas species strains and Acidovorax species by average nucleotide identity(ANI)analysis using OrthoANI and single nucleotide polymorphism(SNP)analysis by snpTree web server.Finally,genomic sequence annotation was conducted with RAST server,as well as functional annotation of orthologous clusters by OrthoVenn,prediction of antibiotic resistance genes(ARGs)by CARD,CRISPRs by CRISPR recognition tool,and prophages by PHAST.These data revealed that C.kerstersii 121606 was a multi-drug resistant bacterium with similar genetic components to other seven Comamonas species and Acidovorax species.The presence of ARGs in its genome helped to explain its multi-drug resistance mechanisms.These findings provide valuable insights for the study of novel antibiotics to manage multi-drug resistant C.kerstersii infections.

关 键 词：Comamonas kerstersii 多药耐药性 比较基因组分析 抗生素耐药性基因 CRISPR 前噬菌体 

分 类 号：Q939.48[生物学—微生物学] R378[医药卫生—病原生物学]