银杏提取物对大鼠蛛网膜下出血后脑神经元氧化应激损伤的保护作用  

Protective effect of Ginkgo biloba extract on oxidative stress injury of brain neurons after subarachnoid hemorrhage in rats

作　　者：管一鸣[1] 谭佳慧[1] GUAN Yi-ming;TAN Jia-hui(Department of Acupuncture and Encephalopathy,Heilongjiang Hospital of Traditional Chinese Medicine,Heilongjiang Academy of Chinese Medicine,Harbin 150036,Heilongjiang Province,China)

机构地区：[1]黑龙江省中医医院、黑龙江省中医药科学院针灸脑病科,黑龙江哈尔滨150036

出　　处：《中国临床药理学杂志》2023年第24期3623-3627,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的 研究银杏提取物对大鼠蛛网膜下出血后脑神经元氧化应激损伤的保护作用及血肿的吸收的影响。方法 将40只雄性SD大鼠随机分成假手术组、模型组和低、高剂量实验组。假手术组10只仅开颅钻孔,其余30只构建蛛网膜下腔出血大鼠模型,每组10只,假手术组和模型组给予等体积0.9%NaCl腹腔注射,低、高剂量实验组分别给予20、100 mg·kg^(-1)·d^(-1)银杏提取物,腹腔注射,均连续治疗14 d。比较各组大鼠神功能缺损评分和脑出血量。以苏木精-伊红(HE)染色观察脑组织损伤情况,以原位末端标记(TUNEL)法计算细胞凋亡率,以酶联免疫吸附法检测脑组织匀浆液白细胞介素(IL)-1、IL-6、IL-1β、肿瘤坏死因子-α(TNF-α)、超氧化物歧化酶(SOD)和丙二醇(MDA)含量。结果 HE病理染色表明,实验组脑组织损伤得到显著保护,脑组织细胞排列整齐,核凋亡数量显著降低,且水肿消失。治疗后,假手术组、模型组和低、高剂量实验组的神经功能缺损评分分别为0、(2.82±0.32)、(2.13±0.53)和(1.33±0.26)分;出血量分别为0、(8.03±1.18)、(5.73±1.06)和(3.47±0.94)mL;神经细胞凋亡率分别为(3.74±0.83)%、(57.70±8.13)%、(26.83±3.14)%和(10.17±1.89)%,组间差异均有统计学意义(均P<0.05)。低、高剂量实验组大鼠脑组织IL-1、IL-6、IL-1β及TNF-α水平均低于模型组大鼠(均P<0.05);低、高剂量实验组大鼠脑组织SOD高于模型组,MDA水平均低于模型组(均P<0.05)。结论 银杏提取物可以有助于保护脑组织,减少氧化应激和蛛网膜下腔对脑组织的损伤。Objective To investigate the protective effect of Ginkgo biloba extract on the oxidative stress injury of brain neurons after subarachnoid hemorrhage and the effect of hematoma absorption in rats.Methods Forty male SD rats were randomly divided into sham operation group,model group and low,high dose experimental groups.Ten rats in sham operation group underwent craniotomy and drilling,while the other30 rats were established rat model of subarachnoid hemorrhage,each group 10 rats.Both the sham operation group and model group were given intraperitoneal injection of 0.9% NaCl in equal amounts,low and high dose experimental groups were given 20 and 100 mg·kg^(-1)·d^(-1) Ginkgo biloba extract via intraperitoneal injection continuously for 14 d.The neurological deficit scores and the level of cerebral hemorrhage for each rat group were compared.Brain tissue damage was observed by hematoxylin and eosin(HE) staining.Cell apoptosis rate was evaluated by terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL) method.Furthermore,the presence of interleukin(IL)-1 and IL-6,IL-1β,tumor necrosis factor-α(TNF-α),superoxide dismutase(SOD) and malondialdehyde(MDA) levels were detected by enzyme-linked immunosorbent assay.Results HE pathological staining revealed that the experimental group exhibited notable protection against brain tissue damage,manifested by orderly arranged brain tissue cells,substantially reduced nuclear apoptosis,and disappearance of edema.The neurological deficit scores after treatment in sham operation group,model group and low,high dose experimental groups were 0,2.82±0.32,2.13±0.53 and 1.33±0.26;the bleeding volume were 0,(8.03±1.18),(5.73±1.06) and(3.47±0.94) mL;the apoptosis rates of nerve cells were(3.74±0.83) %,(57.70±8.13) %,(26.83±3.14) % and(10.17±1.89) %,respectively,all with statistically significant differences(all P <0.05).Levels of IL-1,IL-6,IL-1β and TNF-α in the brain tissue of low and high dose experimental groups of rats were significantly lower than those

关 键 词：银杏提取物 蛛网膜下出血 脑神经元 氧化应激 

分 类 号：R28[医药卫生—中药学]