岩藻多糖对系统性红斑狼疮小鼠肾损伤的影响  

Effect of fucoidan on kidney injury in mice with systemic lupus erythematosus

作　　者：孟瑞玲 李惠丽 张志成 许银怀[3] MENG Rui-ling;LI Hui-li;ZHANG Zhi-cheng;XU Yin-huai(Department of Medical Technology,Puyang Medical College,Puyang 457000,Henan Province,China;Department of Obstetrics and Gynecology,Puyang Maternal and Child Health Hospital,Puyang 457000,Henan Province,China;Puyang Center for Disease Control and Prevention,Puyang 457001,Henan Province,China)

机构地区：[1]濮阳医学高等专科学校医学技术系,河南濮阳457000 [2]濮阳市妇幼保健院产科,河南濮阳457000 [3]濮阳市疾病预防控制中心检验科,河南濮阳457001

出　　处：《中国临床药理学杂志》2023年第24期3638-3642,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的 研究岩藻多糖对系统性红斑狼疮(SLE)小鼠肾损伤的保护作用及其作用机制。方法 将雌性MRL/lpr小鼠随机分为模型组、阳性对照组、低剂量实验组、高剂量实验组,每组10只,另选10只雌性MRL/MpJ小鼠作为对照组;其中低、高剂量实验组小鼠分别每天灌胃给予150、300 mg·kg^(-1)岩藻多糖,阳性对照组小鼠每天灌胃给予5 mg·kg^(-1)泼尼松,对照组和模型组小鼠每天灌胃给予等量的0.9%NaCl。给药4周后采集血液和胸腺、脾、肾器官组织保存备用。以苏木精-伊红(HE)染色观察各组小鼠肾组织病理学变化;以试剂盒检测血清尿素氮(BUN)、血清肌酸酐(Scr)水平和血清炎症因子水平;以免疫组化法和蛋白质印迹(Western blot)法检测肾组织Nod样受体蛋白3(NLRP3)和胱天蛋白酶-1(caspase-1)表达水平。结果 对照组、模型组、低剂量实验组、高剂量实验组、阳性对照组小鼠脾指数分别为4.56±0.24、2.41±0.38、2.91±0.23、3.70±0.35和3.12±0.51;BUN水平分别为(4.02±0.57)、(11.26±1.60)、(9.38±0.63)、(7.73±0.42)和(7.09±0.70)mmol·L^(-1);白细胞介素-18(IL-18)水平分别为(15.11±1.60)、(62.45±7.45)、(42.64±5.76)、(23.88±2.96)和(30.65±2.77)ng·L^(-1);以上指标,模型组与对照组比较,低剂量实验组、高剂量实验组、阳性对照组分别与模型组比较,高剂量实验组与低剂量实验组比较,差异均有统计学意义(均P<0.05)。结论 岩藻多糖可改善SLE小鼠肾组织病理学变化,调节免疫功能,控制炎症进而提高肾功能,这可能与抑制NLRP3炎性小体有关。Objective To investigate the protective effects of fucoidan on renal injury in systemic lupus erythematosus(SLE) mice and its mechanism.Methods Female MRL/lpr mice were randomly divided into model group,low dose experimental group,high dose experimental group and positive control group,with 10 mice in each group.Another10 female MRL/MpJ mice were selected as control group.Mice in low dose experimental and high dose experimental groups were intragastrically given 150 and 300 mg · kg^(-1) fucoidan,mice in positive control group were intragastrically given 5 mg · kg^(-1) of prednisone,mice in control group and model gr oup were intragastrically given the same amount of normal saline.After 4 weeks of administration,blood and organs of thymus,spleen and kidney were collected and stored for later use.The histopathological changes of kidney were observed by hematoxylin-eosin(HE) staining.Serum blood urea nitrogen(BUN) and serum creatinine(Scr) levels were detected by the kit.Serum inflammatory factors were detected by kit method.The expression levels of Nod-like receptor protein 3(NLRP3) and caspase-1 in renal tissues were determined by immunohistochemistry and Western blot.Results The spleen index of mice in control group,model group,low dose experimental group,high dose experimental group and positive control group were 4.56±0.24,2.41±0.38,2.91±0.23,3.70±0.35 and 3.12±0.51;BUN levels were(4.02±0.57),(11.26±1.60),(9.38±0.63),(7.73±0.42) and(7.09±0.70) mmol·L^(-1);IL-18 levels werc(15.11±1.60),(62.45±7.45),(42.64±5.76),(23.88±2.96) and(30.65±2.77) ng·L^(-1),respectively.The above indicators,model group compared with control group;low dose experimental group,high dose experimental group and positive control group were compared with model group,respectively;high dose experimental group compared with low dose experimental group,the differences were all statistically significant(all P <0.05).Conclusion Fucoidan can improve the histopathological changes of kidney in SLE mice,regulate immune function,contro

关 键 词：岩藻多糖 统性红斑狼疮 肾损伤 炎症反应 Nod样受体蛋白3炎性小体 

分 类 号：R28[医药卫生—中药学]