基于转录组信息的杜梨盐胁迫相关MYB转录因子鉴定与分析  

Identification and Analysis of MYB Transcription Factors Related to Salt Stress in Pyrus betulaefolia based on Transcriptome Information

作　　者：李慧[1] 张雨峰 李晓刚[1] 王中华[1] 常有宏[1] 蔺经[1] LI Hui;ZHANG Yu-Feng;LI Xiao-Gang;WANG Zhong-Hua;CHANG You-Hong;LIN Jing(Institute of Pomology/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室,南京210014 [2]南京林业大学生物与环境学院,南京210037

出　　处：《农业生物技术学报》2024年第1期132-146,共15页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金面上项目(31772287);江苏省自然科学基金(BK20191238);江苏省种业振兴揭榜挂帅项目【JBGS〔2021〕084】;江苏现代农业产业技术体系建设项目【JATS[2022]438】。

摘　　要：MYB(myeloblastosis)蛋白是植物中最大的转录因子家族之一,广泛参与植物逆境胁迫应答。到目前为止,对于同一物种不同耐盐能力生态型之间MYB家族的表达模式还没有系统的比较分析。本研究基于杜梨(Pyrus betulaefolia)耐盐单株和普通单株转录组测序(RNA-sequencing,RNA-Seq)数据,筛选鉴定杜梨盐胁迫前后差异表达MYB基因,进行保守结构域归类、染色体分布分析、亚细胞定位预测和系统进化树构建,检测盐胁迫后PbMYBs基因在杜梨不同耐盐性株系各器官中的表达情况。借助转录组分析工具,注释获得129个盐胁迫后差异表达的杜梨PbMYBs基因,根据其结构特性可分为3大类:1RMYB、R2R3-MYB和3R-MYB。杜梨15号染色体上分布的PbMYBs基因数目最多。构建系统进化树发现,杜梨MYB家族包括3个大类、23个亚类。亚细胞定位预测结果显示,42个PbMYBs转录因子定位于胞外,87个定位于细胞核中。基于转录组数据的基因表达模式分析表明,杜梨PbMYBs基因参与了不同器官应对盐胁迫的转录调控;荧光定量PCR证实,不同PbMYBs转录因子基因分别在杜梨根、茎或叶中受盐胁迫信号诱导表达水平上调或下调。上述结果为进一步研究杜梨MYB家族的基因结构和生物学功能提供相关资料。Myeloblastosis(MYB)is one of the most common transcription factor families in plants,which widely takes part in various adversities responses including salt stress.So far,there is still no systematic comparative analysis on the expression pattern of MYB family among various ecotypes with different salt tolerance abilities from the same species.Based on the transcriptome sequencing(RNA-Seq)data from the salt-tolerant ecotype and common ecotype of Pyrus betulaefolia,the differentially expressed PbMYBs genes before and after salt stress were screened and annotated in this study.Then,their conserved domains were classified,subcellular localizations were predicted and the phylogenetic tree was constructed.TBtools was used to draw the heatmaps to analyze the differential expression pattern of PbMYBs genes in roots,stems and leaves from the salt-tolerant ecotype and common ecotype of P.betulaefolia.By using transcriptome analysis tools,129 PbMYBs transcription factors were annotated and selected from the RNA-Seq data,which differentially expressed in 2 ecotypes of P.betulaefolia after salt stress.These genes can be divided into 3 categories(1R-MYB,R2R3-MYB and 3R-MYB)according to their structural characteristics.The number of PbMYBs genes distributed on chromosome 15 of P.betulaefolia was the largest.The phylogenetic trees of MYB families were constructed,which showed that the PbMYBs family of P.betulaefolia contained 3 major branches,and 23 evolutionary branches.The results of the subcellular localization demonstrated that 42 PbMYBs were located in the extracellular,and 87 PbMYBs were located in the nucleus.Analysis of the differential expression pattern of PbMYBs based on transcriptome data showed that PbMYBs genes in P.betulaefolia may be involved in the transcriptional regulation of different organs in response to salt stress.Additionally,the results of real-time quantitative PCR further verified that some PbMYBs transcription factors may play a role in stress regulation in the roots,stems or leaves of P.betulaefoli

关 键 词：杜梨 盐胁迫 转录组 MYB转录因子 表达模式 

分 类 号：S661.2[农业科学—果树学]