拟南芥C2H2型转录因子AtIDD5结构和功能的生物信息学分析  

Bioinformatic analysis of structure and function of type C2H2 transcription factor AtIDD5 in Arabidopsis

作　　者：徐晓东 张国斌 李林倩 陈健鑫 于德嘉 董萍萍 殷伊君 郭丽红 杨红玉 XU Xiao-dong;ZHANG Guo-bin;LI Lin-qian;CHEN Jian-xin;YU De-jia;DONG Ping-ping;YIN Yi-jun;GUO Li-hong;YANG Hong-yu(College of Agronomy and Life Sciences,Kunming University,Kunming 650214,China;College of Plant Protection,Yunnan Agricul-tural University,Kunming 650201,China;College of Biodiversity Conservation,Southwest Forestry University/Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province,Kunming 650224,China;School of Biological Resources and Food Engi-neering,Qujing Normal University,Qujing,Yunnan 655011,China)

机构地区：[1]昆明学院农学与生命科学学院,昆明650214 [2]云南农业大学植物保护学院,昆明650201 [3]西南林业大学生物多样性保护学院/云南省高校森林灾害预警控制重点实验室,昆明650224 [4]曲靖师范学院生物资源与食品工程学院,云南曲靖655011

出　　处：《西南农业学报》2023年第11期2319-2328,共10页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31760078);云南省教育厅研究生项目(2022Y700,2023Y0862);昆明学院人才引进项目(YJL18002)。

摘　　要：【目的】研究拟南芥C2H2型转录因子AtIDD5结构和功能的生物信息,分析其理化性质、组织表达模式、亚细胞定位、蛋白互作网络和启动子顺式作用元件,预测AtIDD5同源蛋白、参与响应元件以及AtIDD5调控与被调控基因,为进一步研究AtIDD5蛋白在植株生长发育中的作用机制提供线索。【方法】利用Uniprot、TMHMM2.0、MEGA7.0、STRING等软件对AtIDD5蛋白进行理性化性质推断、AtIDD5基因在植物组织中的表达模式预测、AtIDD5蛋白进行亚细胞定位分析、蛋白互相作用网络构建以及AtIDD5基因启动子顺式作用元件分析。【结果】AtIDD5基因的编码区(Coding sequence,CDS)全长1809 bp,编码602个氨基酸,其中丝氨酸占比最高,为17.5%。根据所含元素的数量推算其分子式为C1475 H2273 N469 O528 S12,不稳定指数为56.63,属于不稳定蛋白。AtIDD5蛋白含有4个锌指结构,属于典型的C2H2型转录因子。构建AtIDD5同源蛋白序列系统发育树,其中氨基酸序列相似度大于60%的其他物种有6个,分别是亚麻芥(Camelina sativa)、盐芥(Eutrema salsugineum)、白芥(Sinapis alba)、油菜(Brassica na⁃pus)、萝卜(Raphanus sativus)、芝麻菜(Eruca vesicaria subsp)。基因表达模式预测AtIDD5基因在茎、叶、花、角果、花中均有表达,在叶片中的表达量最高;亚细胞定位预测AtIDD5蛋白位于细胞核与叶绿体中。蛋白互作网络预测了与AtIDD5互作的蛋白有20个,直接结合的有10个。AtIDD5蛋白通过与多种蛋白的互作广泛参与了植物生长发育基因表达的调控过程。AtIDD5基因启动子包含有大量的核心顺式元件、光响应元件和激素响应元件。【结论】推测AtIDD5基因本身的表达受到光和激素等因子的调控;预测AtIDD5基因在植株生长发育、激素调节和逆境胁迫中发挥着重要作用。【Objective】To study the bioinformatics role of the structure and function of the type C2H2 transcription factor AtIDD5 in biological procession,its physicochemical properties,tissue expression patterns,subcellular localization,protein interaction network,and promoter cis⁃acting elements were analyzed.The homologous proteins of AtIDD5,its participation in response elements,and the regulation of AtIDD5 and its regulated genes were presumed.The study could provide clues for further investigation into the mechanism of AtIDD5 protein in plant growth and development.【Method】Bioinformatics analysis of AtIDD5 protein was performed using software such as Uniprot,TMHMM2.0,MEGA7.0,STRING,etc.The physicochemical properties and promoter cis⁃acting elements of AtIDD5 gene,tissue expression patterns and subcellular localization of AtIDD5 protein,and protein interaction network were analyzed.【Result】The study revealed that the coding se⁃quence(CDS)of the AtIDD5 gene was 1809 bp,which encoded 602 amino acids,and serine accounted for the highest proportion of 17.5%.According to the number of elements contained,its molecular formula was C1475 H2273 N469 O528 S12,and the instability index was 56.63,which was an unstable protein.AtIDD5 contained four zinc finger structures and was a typical C2H2 transcription factor.The AtIDD5 homologous protein sequence phylogenetic tree was constructed,in which there were 6 other species with amino acid sequence simi⁃larity greater than 60%,Camelina sativa,Eutrema salsugineum,Sinapis alba,Brassica napus,Raphanus sativus and Eruca vesicaria subsp.The predicted expression pattern indicated that the AtIDD5 gene was expressed in the stem,leaf,flower and silique,with the highest expres⁃sion in the leaf.The subcellular localization analysis showed that the AtIDD5 protein was localized in the nucleus and chloroplasts.The pro⁃tein interaction network predicted 20 proteins interacting with AtIDD5,10 of which were direct interactions.These data suggested that AtIDD5 played a widesprea

关 键 词：AtIDD5 生物信息学 亚细胞定位 表达模式 互作蛋白 启动子分析 

分 类 号：S312[农业科学—作物栽培与耕作技术]