一株厚壁隔孢伏革菌的筛选及木质纤维素降解特性  

Screening of a Peniophora crassitunicata and its lignocellulosic degradation characteristics

作　　者：陈俊文 刘庆华[1] 庞学勇[1] CHEN Jun-wen;LIU Qing-hua;PANG Xue-yong(Chengdu Institute of Biology,Chinese Academy of Sciences/CAS Key Laboratory of Mountain Ecological Restoration and Bioresource U-tilization/Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province,Chengdu 610041,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院成都生物研究所/中国科学院山地生态恢复与生物资源利用重点实验室/生态恢复与生物多样性保育四川省重点实验室,成都610041 [2]中国科学院大学,北京100049

出　　处：《西南农业学报》2023年第11期2491-2499,共9页Southwest China Journal of Agricultural Sciences

基　　金：四川省科技计划项目(2022YFS0499);国家自然科学基金项目(32171756)。

摘　　要：【目的】木质素难降解是制约高木质纤维素含量的农林废弃资源利用的重要因素。筛选木质素降解菌可为高效利用农林废弃物提供参考依据。【方法】以愈创木酚显色法和苯胺蓝褪色法从广泛收集的植物凋落物中分离菌株;结合形态和分子生物学方法鉴定菌株;同时测定3种木质素降解酶(漆酶、锰过氧化物酶和木质素过氧化物酶)的活性;为进一步验证菌株的降解能力,选用常见的农林废弃物-葡萄枝条作为材料,分析其在葡萄枝条废弃物液态发酵中对纤维素、半纤维素和木质素成分的降解情况。【结果】从植物凋落物中筛选出一株具有分解木质素特性的菌株HUA。菌株HUA在愈创木酚⁃PDA培养基上呈现明显的红棕色显色圈,在苯胺蓝⁃PDA培养基上最终产生褪色反应。结合形态和分子生物学方法,该菌被鉴定为厚壁隔孢伏革菌(Peniophora crassi⁃tunicata)。该菌株发酵液中漆酶、锰过氧化物酶和木质素过氧化物酶活性分别为53.18、174.01、9.51 U/L。葡萄枝条废弃物液态发酵结果表明,该菌能够有效降解木质纤维素成分,经过发酵20 d后,纤维素、半纤维素和木质素的降解率分别为17.50%、36.55%和10.33%。【结论】本研究获得的菌株HUA促进了葡萄枝条木质纤维素成分的降解,为木质纤维素高效降解提供了优质菌种资源,同时也为该菌株后续应用研究积累了相关数据。【Objective】The refractory lignin is an important factor restricting the utilization of agricultural and forestry wastes resources with high lignocellulosic content.Screening lignin⁃degrading strain can provide reference for efficient utilization of agricultural and forestry wastes.【Method】In the study,strain was isolated from extensive collection of plant litter materials by using guaiacol chromogenic method and aniline blue fading method.The enzyme activities of three lignin⁃degrading enzymes(laccase,manganese peroxidase and lignin peroxidase)were measured.To further verify the degradation ability of the strain,vineyard prunings(common agricultural and forestry wastes)were selected as materials to analyze the degradation of cellulose,hemicellulose and lignin in liquid fermentation of vineyard prunings.【Result】A strain HUA with lignin decomposition characteristic was screened from plant litter.The strain HUA showed obvious reddish⁃brown color circle on guaiacol⁃PDA plate,and finally produced decolorizing reaction on aniline blue⁃PDA plate.Based on morphology and molecular biological methods,it was identified as Peniophora crassitunicata.Three lignin⁃degrading enzymes(laccase,manganese peroxidase and lignin peroxi⁃dase)in fermentation broth of this strain were 53.18,174.01,9.51 U/L,respectively.The results of vineyard prunings liquid fermentation showed that the strain could degrade lignocellulosic components effectively.After 20 days of fermentation,the degradation rates of cellulose,hemicellulose and lignin reached 17.50%,36.55%,10.33%,respectively.【Conclusion】The strain HUA obtained in this study promotes the degradation of lignocellulose components in vineyard prunings,which provides high⁃quality strain resources for the efficient degradation of lignocellulose and accumulated relevant data for subsequent application studies of strain.

关 键 词：厚壁隔孢伏革菌 木质纤维素 酶活 葡萄枝条 农林废弃物 

分 类 号：S816.3[农业科学—饲料科学]