一株茶毒蛾病原细菌的分离、鉴定及致病性测定  

Isolation,identification and pathogenicity test of a pathogenic bacteria of Euproctis pseudoconspersa(Lepidoptera:Lymantriidae)

作　　者：张倪铭 陈李林[2] 倪德芳 李文凯 童应华[1] ZHANG Niming;CHEN Lilin;NI Defang;LI Wenkai;TONG Yinghua(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建农林大学植物保护学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2024年第1期22-28,共7页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：中国白茶研究院开放课题(BCY2022K05)。

摘　　要：【目的】茶毒蛾是一种危害山茶属植物的主要害虫。对茶毒蛾病原细菌进行分离、鉴定及致病性测定,为该虫的生物防治提供依据。【方法】从茶毒蛾病死虫体上分离得到一株病原细菌菌株Nan-Y,对该菌株进行形态观察和16S rDNA分子鉴定,并采用喂食法测定浓度为(1.0±0.5)×10^(10)、(1.0±0.5)×10^(11)、(1.0±0.5)×10^(12)、(1.0±0.5)×10^(13)、(1.0±0.5)×10^(14)cfu·mL^(-1)的菌悬液对茶毒蛾4龄幼虫的毒力。【结果】Nan-Y菌体杆状,在NA培养基上菌落呈淡黄色点状,边缘较整齐,表面黏稠;Nan-Y菌株为不解糖假苍白杆菌(Pseudochrobactrum asaccharolyticum),革兰氏阴性。随着菌悬液浓度增大茶毒蛾幼虫的死亡率不断升高,其中,(1.0±0.5)×10^(14)cfu·mL^(-1)菌悬液处理茶毒蛾6 d后,校正死亡率达100.00%。该细菌处理茶毒蛾幼虫3、6 d的LC50分别为5.23×10^(13)、3.04×10^(9)cfu·mL^(-1)。【结论】Nan-Y菌株对茶毒蛾幼虫有较强的致病力,在该害虫的生物防治中有潜在的应用价值。【Objective】An in vitro pathogenicity test of Euproctis pseudoconspersa,a major pest of Camellia plants,was performed to provide reference for the biological control of E.pseudoconspersa.【Method】A pathogenic bacterium strain named Nan-Y was isolated from the dead body of E.pseudoconspersa larva,and then identified by morphological observation and 16S rDNA.And its toxicity to the 4th instar larvae of E.pseudoconspersa was determined by feeding with(1.0±0.5)×10^(10),(1.0±0.5)×10^(11),(1.0±0.5)×10^(12),(1.0±0.5)×10^(13)or(1.0±0.5)×10^(14)cfu·mL^(-1)bacterial suspension.【Result】Nan-Y turned out to be Gram-negative bacteria with rod shape,and its colony on NA medium was punctiform and pale yellow with regular edge and sticky surface.Strain Nan-Y was identified as Pseudochrobactrum asacchrolyticum by 16S rDNA.The mortality of E.pseudoconspersa larvae increased with higher concentrations of bacterial suspensions,with corrected mortality reaching 100.00%at 6 d after feeding with(1.0±0.5)×10^(14)cfu·mL^(-1)bacterial suspension.The LC50 of Nan-Y to E.pseudoconspersa larvae was 5.23×10^(13)and 3.04×10^(9)cfu·mL^(-1)at 3 and 6 d,respectively.【Conclusion】Strain Nan-Y has strong pathogenicity to E.pseudoconspersa larvae,thus having application potential in the biological control of the pest.

关 键 词：茶毒蛾 病原细菌 致病力 不解糖假苍白杆菌 生物防治 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]