生态因子对三叶木通和五叶木通居群遗传多样性的影响  

Influence of ecological factors on the genetic diversity ofthe population of Akebia trifoliata and Akebia quinata

作　　者：汤腾跃 余柯达[2] 王佳淇 范超洋 TANG Tengyue;YU Keda;WANG Jiaqi;FAN Chaoyang(People′s Government of Yafan Town,Wucheng District,Jinhua City,Jinhua 321000,Zhejiang;Zhejiang Normal University,Jinhua 321004,Zhejiang;Shanghai New Era Wuyi Senior High School,Jinhua 321017,Zhejiang;Jinhua Xianshida Agricultural Technology Co.,Ltd,Jinhua 321004,Zhejiang)

机构地区：[1]金华市婺城区雅畈镇人民政府,浙江金华321000 [2]浙江师范大学,浙江金华321004 [3]上海新纪元武义高级中学,浙江金华321017 [4]金华先实大农业科技有限公司,浙江金华321004

出　　处：《浙江农业科学》2024年第1期66-72,共7页Journal of Zhejiang Agricultural Sciences

摘　　要：采用简单重复序列区间(ISSR)扩增技术和rDNA转录间隔区(ITS)序列分析技术,对浙江省7个地区34个三叶木通和五叶木通个体的遗传多样性进行分析。结果表明,五叶木通的聚类与海拔呈显著相关,而与土壤有机质含量和pH值相关性不大。对三叶木通进行ISSR分析,筛选出15对引物可获得265条条带,其中多态性条带比例是98.49%。聚类分析表明,同一地区的三叶木通各自聚为一类,说明三叶木通在不同地域间遗传差异显著。三叶木通总的Nei′s基因多样性指数(h)为0.503 3,Shannon′s信息指数(I)为0.689 0,表明三叶木通的遗传多样性较高。通过相关性分析表明,海拔、土壤有机质含量和pH值这3种生态因子与三叶木通遗传多样性的相关性不显著。综上,rDNA ITS序列分析和ISSR分子标记分别适用于五叶木通和三叶木通的遗传多样性分析。本研究表明,海拔与五叶木通的遗传多样性显著相关,三叶木通遗传多样性与海拔、土壤有机质含量和pH值相关性不明显,但不同地域间三叶木通种群的遗传差异明显。Genetic diversity of 34 species of Akebia trifoliata(Thunb.)Koidz and Akebia quinata(Thunb.)Decne from seven regions in Zhejiang Province was analyzed using inter simple sequence repeat(ISSR)amplification technology and rDNA internal transcribed spacer(ITS)sequence analysis technology.The results showed that the cluster analysis of Akebia quinata(Thunb.)Decne was significantly correlated with altitude,but less correlated with the soil organic matter content and pH value.The 15 primers selected were used in the ISSR analysis for Akebia trifoliata(Thunb.)Koidz to obtain 265 DNA bands,of which 98.49%bands were polymorphic.The cluster analysis indicated that Akebia trifoliata(Thunb.)Koidz from the same region could be classified as one cluster respectively.And the genetic differences were significant in different regions.The mean Nei′s gene diversity index(h)of Akebia trifoliata(Thunb.)Koidz was 0.5033 and Shannon′s information diversity index(I)was 0.6890,which showed rich genetic diversity of Akebia trifoliata(Thunb.)Koidz.The correlation analysis showed that it wasn′t significantly correlated between the three ecological factors of altitude,soil organic matter content and pH value and genetic diversity of Akebia trifoliata(Thunb.)Koidz.In summary,rDNA ITS sequence analysis and ISSR molecular marker were applied separately to genetic diversity analysis of Akebia trifoliata(Thunb.)Koidz and Akebia quinata(Thunb.)Decne.The study indicates that altitude was significantly correlated with genetic diversity of Akebia quinata(Thunb.)Decne.Genetic diversity of Akebia trifoliata(Thunb.)Koidz wasn′t significantly correlated with altitude,soil organic matter content and pH value,but genetic differences of Akebia trifoliata(Thunb.)Koidz in different regions was significant.

关 键 词：三叶木通 五叶木通 ITS ISSR 生态因子 遗传多样性 

分 类 号：S326[农业科学—作物遗传育种]