番茄液泡蔗糖转化酶抑制蛋白克隆、生物信息学及表达分析  

Molecular cloning, bioinformatics and expression analysis ofvacuolar invertase inhibitor in tomato

作　　者：许洋 程远[2] 阮美颖[2] 王荣青[2] 叶青静[2] 姚祝平[2] 周国治[2] 刘乐承[1] 万红建[2] XU Yang;CHENG Yuan;RUAN Meiying;WANG Rongqing;YE Qingjing;YAO Zhuping;ZHOU Guozhi;LIU Lecheng;WAN Hongjian(College of Horticulture and Landscape Architecture,Yangtze University,Jingzhou 434000,Hubei;Institute of Vegetable,Zhejiang Academy of Agricultural Sciences/State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/China-Australia Research Centre for Crop Improvement,Hangzhou 310021,Zhejiang)

机构地区：[1]长江大学园艺园林学院,湖北荆州434000 [2]农产品质量安全危害因子与风险防控国家重点实验室浙江省农业科学院蔬菜研究所中澳作物改良中心,浙江杭州310021

出　　处：《浙江农业科学》2024年第1期81-89,共9页Journal of Zhejiang Agricultural Sciences

基　　金：浙江省重点研发计划项目(2021C02052);国家重点研发计划(2018YFD1000800);国家自然科学基金面上项目(32172555,31772294);浙江省农业(蔬菜)新品种选育重大科技专项(2021C02065);国家现代农业产业技术体系资助项目(CARS-23-G44)。

摘　　要：液泡转化酶抑制蛋白(vacuolar invertase inhibitor, VIF)在调节植物蔗糖代谢,调控生长发育及响应逆境等方面发挥重要作用。本试验从番茄Alisa中克隆到VIF基因(SlVIF)和cDNA序列。结果显示,SlVIF基因与cDNA序列全长均为528 bp,不含有内含子,共编码175个氨基酸。SlVIF蛋白分子量为19.92 ku,理论等电点为5.53,含有一个信号肽序列,保守结构域为PMEI。系统进化关系表明,茄科物种的VIF亲缘关系最近,且基序具有一致性。组织特异性表达结果显示,在不同的番茄材料中,SlVIF的表达模式存在差异,在Heinz番茄中,其在根部有高水平的表达;在LA1589番茄中,其在果实发育的各阶段,尤其是在成熟果实及根部有高水平的表达。说明SlVIF可能通过介导蔗糖代谢影响果实成熟并调控根系生长。SlVIF受到水分胁迫的诱导,响应非生物胁迫过程。Vacuolar invertase inhibitor(VIF)plays an important role in regulating higher plant sucrose metabolism and responding to abiotic stress.In this study,VIF gene and cDNA sequence were cloned from Alisa.The results indicated that the full length of SlVIF gene and cDNA was 528 bp,without intron region,encoding 175 amino acids.The molecular weight of SlVIF protein was 19.92 ku,and the theoretical isoelectric point was 5.53.It contained a signal peptide sequence,and the conservative domain was PMEI.The phylogenetic relationship exhibited that the VIF of Solanaceae species was the closest and the motifs were consistent.The tissue specific expression results showed that it had different expression patterns in various tomato materials.In Heinz,it had high transcription level in roots.In LA1589,it was highly expressed in all stages of fruit development,especially in mature fruits and roots,which indicated that SlVIF gene may play a role in influencing fruit ripening and regulating root growth by mediating sucrose metabolism.SlVIF was induced by water stress and responded to abiotic stress processes.

关 键 词：液泡转化酶抑制蛋白 番茄 蔗糖代谢 表达 

分 类 号：S641.2[农业科学—蔬菜学]