外源GA3和ABA对烤烟内源激素调控效应和常规化学成分的影响  

Effects of exogenous GA 3 and ABA on endogenous hormone regulationand conventional chemical composition of flue-cured tobacco

作　　者：李波 陈小翔[1] 张文军 翟争光 刘化冰[1] 江智敏[1] 徐志强[1] LI Bo;CHEN Xiaoxiang;ZHANG Wenjun;ZHAI Zhengguang;LIU Huabing;JIANG Zhimin;XU Zhiqiang(Zhejiang Zhongyan Industry Co.,Ltd.Technology Development Branch,Hangzhou 310008,Zhejiang;Hunan Tobacco Company Changsha Company,Changsha 410000,Hunan)

机构地区：[1]浙江中烟工业有限责任公司技术开发分公司,浙江杭州310008 [2]湖南省烟草公司长沙市公司,湖南长沙410000

出　　处：《浙江农业科学》2024年第1期181-189,共9页Journal of Zhejiang Agricultural Sciences

基　　金：中国烟草总公司河南省公司重点项目(2021410000240021);河南省烟草公司许昌市公司重点项目(2020411000240069);贵州省烟草公司贵阳市公司重点项目;湖南省烟草公司长沙市公司重点项目(21-23A04);广西壮族自治区烟草公司百色市公司重点项目(202145100020165);福建省烟草公司南平市公司重点项目(NYK2021-09-03)。

摘　　要：本文从激素和基因水平探究外源激素赤霉素(GA3)和脱落酸(ABA)的作用机理,研究其对烤烟生长发育及产量和质量的影响,为田间施用外源激素提供理论依据。采用大田试验,材料为中烟100(ZY100)和NC71,喷施不同浓度配比的GA3和ABA,测定不同时期烟株的农艺性状、基因表达量、生理特性、内源激素含量及烤后烟叶化学成分。结果表明,与未喷施GA3处理相比,喷施GA3后ZY100株高升高20%,NC71株高升高31%;喷施ABA后ZY100株高降低17%,NC71株高降低10%。喷施GA3后ZY100和NC71内源赤霉素含量分别升高20%和30%;喷施外源GA3后ZY100和NC71烟株内CPS、GA20ox和PSY的基因表达量均上调,GA2ox基因表达量下调。喷施GA3提高了ZY100和NC71烟株体内叶绿素以及可溶性蛋白的含量,降低了丙二醛含量,提高了烤后烟叶总糖含量、钾含量、钾氯比,降低了烟碱含量和氯含量,显著提高了烤烟的经济性状。In this paper,the mechanism of exogenous hormones gibberellin(GA 3)and abscisic acid(ABA)was investigated from the hormone and gene levels,and the effects of exogenous hormones on the growth and development,yield and quality of flue-cured tobacco were studied,providing theoretical basis for field application of exogenous hormones.Field experiments were conducted with Zhongyan100 and NC71,and different concentrations of GA 3 and ABA were sprayed to determine the agronomic traits,gene expression,physiological characteristics,endogenous hormone content and chemical composition of tobacco leaves after roasting.The results showed that the height of ZY100 and NC71 plants increased by 20%and 31%respectively after GA 3 spraying compared with no GA 3 spraying.After spraying ABA,the height of ZY100 plant decreased by 17%,and the height of NC71 plant decreased by 10%.After spraying GA 3,the content of endogenous gibberellin in ZY100 and NC71 increased by 20%and 30%,respectively.After spraying exogenous GA 3,the gene expressions of CPS,GA20ox and PSY in ZY100 and NC71 tobacco plants were up-regulated,while the gene expressions of GA2ox were down-regulated.Spraying GA 3 increased the contents of chlorophyll and soluble protein in ZY100 and NC71,decreased the content of malondialdehyde,increased the total sugar content,potassium content,potassium chlorine ratio,decreased the nicotine content and chlorine content,and significantly improved the economic properties of flue-cured tobacco.

关 键 词：赤霉素 脱落酸 烤烟 基因表达 生理特性 常规化学成分 

分 类 号：S572[农业科学—烟草工业]