白芍多糖对肝阴虚证模型大鼠证候表征及内分泌/能量代谢的影响  

The effect of Polysaccharides of Radix Paeoniae Alba on the syndrome characterization and endocrine/energy metabolism of liver Yin deficiency model rats

作　　者：高晶 范晓旭 冯颖童 王祯 沈奕玮 李伟 王景霞 GAO Jing;FAN Xiaoxu;FENG Yingtong;WANG Zhen;SHEN Yiwei;LI Wei;WANG Jingxia(School of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学中医学院,100029

出　　处：《环球中医药》2024年第1期2-10,共9页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(82074036)。

摘　　要：目的在建立肝阴虚证病证结合实验模型的基础上,研究白芍多糖对大鼠化学性肝损伤肝阴虚证证候表征及内分泌/能量代谢的影响。方法SPF级雄性SD大鼠,体质量(200±20)g,适应性喂养一周后,根据体质量随机区组法分为空白组、模型组、一贯煎组、白芍多糖组。除空白组外,其余各组大鼠腹腔注射浓度20%四氯化碳(carbon tetrachloride,CCl 4)橄榄油溶液,联合甲状腺片30 mg/kg同时灌胃给药,连续6周。6周后观察大鼠体质量、易激惹程度、毛发光泽度、小便颜色、大便颜色及质地等并进行评分,测量体质量、肛温、舌面干湿度、摄食量、摄水量,测定血清环磷酸腺苷(cyclic adenosine monophosphate,cAMP)、环磷酸鸟苷(cyclic guanosine monophosphate,cGMP)含量,计算cAMP/cGMP比值。测定血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aliquot aminotransferase,AST)、γ-谷氨酰转肽酶(γ-glutamyl transpeptidase,γ-GT)、碱性磷酸酶(alkaline phosphatase,ALP)、总胆红素(total bilirubin,TBIL)、总胆汁酸(total bile acid,TBA)的含量变化并取肝脏进行苏木精—伊红染色病理切片检查;测定血清睾酮(testosterone,T)、雌二醇(estradiol,E 2)、皮质类固醇(crticosteroid,COR)水平,测定肝脏Na^(+)-K^(+)-ATP酶、Ca^( 2+)-Mg ^(2+)-ATP酶活性。结果与模型组相比,白芍多糖组大鼠大便评分升高(P<0.01),其余体征评分均显著下降(P<0.01);白芍多糖组体质量均有所上升,但差异无统计学意义(P>0.05);实验第2、4周,白芍多糖组肛温下降(P<0.01);白芍多糖组舌面干湿度上升(P<0.01),24小时摄食量、饮水量无统计学差异(P>0.05);白芍多糖组血清cAMP降低,cGMP升高,cAMP/cGMP下降(P<0.01),ALT、AST、γ-GT、ALP、TBIL均下降(P<0.01),TBA含量降低但无统计意义(P>0.05);病理染色显示肝组织结构中度异常,视野内部分肝细胞轻度脂肪变性,胞质内可见大量小空泡,部分肝细胞气球样变性,肝实质内少量炎�Objective Based on establishing a combined experimental model of liver Yin deficiency syndrome,to study the effect of Polysaccharides of Radix Paeoniae Alba(PRAP)on the characterization of liver Yin deficiency syndrome and endocrine energy metabolism in rats with chemical liver injury.Methods SPF male SD rats,weighing(200±20)g,were randomly divided into blank group,model group,Yiguanjian group,and PRAP group after one week of adaptive feeding.Except for the blank group,rats in all other groups were intraperitoneally injected with a concentration of 20%CCl 4 olive oil solution and orally administered with 30 mg/kg thyroid tablets for 6 consecutive weeks.After 6 weeks,the levels of body weight,irritability degree,hair gloss,urine color,stool color and texture of the rats were observed and scored.Body weight,anal temperature,tongue surface humidity,food intake,water intake,serum cyclic adenosine monophosphate(cAMP),cyclic guanosine monophosphate(cGMP)content and cAMP/cGMP were measured.The contents of serum alanine aminotransferase(ALT),aliquot aminotransferase(AST),γ-glutamyl transpeptidase(γ-GT),alkaline phosphatase(ALP),total bilirubin(TBIL),and total bile acid(TBA)were measured.Liver was taken for HE pathology section examination.Serum testosterone(T),estradiol(E 2)and crticosteroid(COR)levels were measured,and the activities of Na^(+)-K^(+)-ATPase and Ca ^(2+)-Mg^( 2+)-ATPase in the liver were detected.Results Compared with the model group,the PRAP group showed an increase in fecal score(P<0.01),while the other physical sign scores were significantly reduced(P<0.01);the body mass of rats in the PRAP group was increased,but the difference was not statistically significant(P>0.05).In the second and fourth week of the experiment,the anal temperature in the PRAP group was decreased(P<0.01);dryness and humidity of the tongue in the PRAP group was increased(P<0.01),but there was no statistically significant difference in 24-hour food intake and water consumption(P>0.05);the PRAP group showed a decrease in serum cA

关 键 词：白芍多糖 化学性肝损伤 肝阴虚证 内分泌 能量代谢 

分 类 号：R285.5[医药卫生—中药学]