基于指纹图谱结合化学模式识别对狗脊药材的质量评价  

Evaluation of quality markers of Cibotii Rhizoma based on fingerprint and chemical pattern recognition

作　　者：贾天宁 刘芳[1,2] 陈鹏 李先宽 马琳[3] 黄钦华[4] JIA Tian-ning;LIU Fang;CHEN Peng;LI Xian-kuan;MA Lin;HUNG Qin-huang(First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300381,China;National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300381,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;The Second Affiliated Hospital of Xiamen Medical College,Xiamen 361021,China)

机构地区：[1]天津中医药大学第一附属医院,天津300381 [2]国家中医针灸临床医学研究中心,天津300381 [3]天津中医药大学,天津301617 [4]厦门医学院附属第二医院,福建厦门361021

出　　处：《中草药》2023年第24期8214-8221,共8页Chinese Traditional and Herbal Drugs

基　　金：国家中药标准化项目(ZYBZH-C-QIN-46);天津市道地药材生态种植及质量保障项目(2022)。

摘　　要：目的采用指纹图谱结合聚类分析(hierarchical clustering analysis,HCA)、主成分分析(principal component analysis,PCA)和偏最小二乘法-判别分析(partial least squares discriminant analysis,PLS-DA)等进行化学模式识别,对狗脊药材指纹图谱数据进行分析,筛选特征性指标成分并以此建立狗脊有效成分的定量分析,为狗脊质量控制提供科学依据。方法采用Agilent Eclipse XDB-C18柱(250 mm×4.6mm,5.0μm),以甲醇-1%醋酸水溶液为流动相进行梯度洗脱,体积流量为1.0 mL/min,柱温为30℃,检测波长为280 nm,HPLC法建立9个产地16批样品狗脊的指纹图谱,运用相似度分析、HCA、PCA和PLS-DA等化学模式识别技术筛选出不同产地狗脊化学成分的特征成分作为狗脊有效成分,并定量分析。结果16批狗脊HPLC指纹图谱标定10个共有峰,相似度在0.695~0.954;通过HCA、PCA和PLS-DA较好的区分各产地狗脊,综合分析筛选5-羟甲基糠醛、原儿茶酸、原儿茶醛、紫云英苷4个成分作为狗脊有效成分,质量分数分别在0.0228%~1.3394%、0.0062%~0.7133%、0.0200%~0.2345%、0.0112%~0.1065%。特征图谱和含量测定结果显示广西产地狗脊药材质量较优。结论该研究建立的狗脊药材指纹图谱及含量测定方法稳定、可靠,重现性好,可为全面评价狗脊药材质量提供参考。Objective To analyze the fingerprint data of Gouji(Cibotii Rhizoma,CR)using chemical pattern recognition technology,screen the characteristic index components and conduct quantitative analysis,hoping to provide scientific basis for the quality control of CR.Methods HPLC was used to establish fingerprints of 16 batches of CR samples from nine producing areas.Chemical pattern recognition techniques such as similarity analysis,cluster analysis,principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA)were used to screen out the characteristic chemical components of CR among different regions as quality markers,and the quantitative analysis was carried out.Results A total of ten common peaks were identified from HPLC fingerprints of 16 batches of CR samples,and the similarity was between 0.695—0.954.A variety of chemical pattern recognition techniques were used to distinguish CR from different producing areas.After comprehensively analysis,four components including 5-HMF,protocatechuic acid,protocatechualdehyde and astragalin were screened out as Q-Markers of CR,and the mass fractions were 0.022 8%—1.339 4%, 0.006 2%—0.713 3%, 0.020 0%—0.234 5%, 0.011 2%—0.106 5%, respectively. The results of characteristic fingerprint and content determination showed that the quality of CR from Guangxi was superior. Conclusion The fingerprint and content determination method established in this study are stable, reliable and reproducible, which can provide a reference for the comprehensive evaluation of the quality of CR.

关 键 词：狗脊 指纹图谱 相似度 化学模式识别 5-羟甲基糠醛 原儿茶酸 原儿茶醛 紫云英苷 

分 类 号：R286.2[医药卫生—中药学]