基于功能模型的痹祺胶囊药效研究  

Efficacy of Biqi Capsule based on functional animal models

作　　者：李新 韩彦琪[1,2,3] 卜睿臻 王春芳 王磊 姚鹏飞 徐旭[1,2,3] 许浚[1,2,3] 赵专友[1,2,3] 张铁军[1,2,3] 刘昌孝[1,2,3] LI Xin;HAN Yan-qi;BU Rui-zhen;WANG Chun-fang;WANG Lei;YAO Peng-fei;XU Xu;XU Jun;ZHAO Zhuan-you;ZHANG Tie-jun;LIU Chang-xiao(Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;National&Local United Engineering Laboratory of Modern Preparation and Quality Control Technology of Traditional Chinese Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;National Key Laboratory of Druggability Evaluation and Systematic Translational Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;Tianjin Darentang Jingwanhong Pharmaceutical Co.,Ltd.,Tianjin 300112,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Tianjin Pharmaceutical Da Ren Tang Group Co.,Ltd.,Tianjin 300193,China)

机构地区：[1]天津药物研究院,天津市中药质量标志物重点实验室,天津300462 [2]天津药物研究院,中药现代制剂与质量控制技术国家地方联合工程实验室,天津300462 [3]天津药物研究院,药物成药性评价与系统转化全国重点实验室,天津300462 [4]天津达仁堂京万红药业有限公司,天津300112 [5]天津中医药大学,天津301617 [6]津药达仁堂集团股份有限公司,天津300193

出　　处：《中草药》2023年第23期7630-7637,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金重点项目(U21A20406)。

摘　　要：目的探讨痹祺胶囊全方益气养血、活血通络、抗炎及镇痛作用。方法分别采用大鼠气血两虚模型和微循环障碍模型观察痹祺胶囊的益气养血和活血化瘀作用;采用小鼠急性耳肿模型观察痹祺胶囊的抗炎作用;采用小鼠热板法和扭体模型观察痹祺胶囊的镇痛作用。各功能研究用动物分别分为空白组(扭体实验除外)、模型组(热板实验除外)、阳性药组、痹祺胶囊低剂量组(相当于临床用量)、中剂量组(相当于临床2倍用量)、高剂量组(相当于临床4倍用量)。末次给药1 h后取材,检测全血中外周血细胞数量,采用ELISA法检测血清中促红细胞生成素(erythropoietin,EPO)含量,采用流式细胞术检测脾脏中CD4^(+)/CD8^(+)比例,用于初步评价痹祺胶囊的益气养血作用;采集大鼠肠系膜毛细血管流速,对全血进行黏度分析,采用凝血酶时间(thrombin time,TT)试剂盒对血清进行凝血时间分析,用于初步评价痹祺胶囊的活血化瘀作用。结果痹祺胶囊低剂量组(相当于临床剂量)具有显著的益气养血及抗炎镇痛作用(P<0.05、0.01),活血化瘀作用不明显;痹祺胶囊中剂量(相当于2倍临床剂量)表现出显著的抗炎及镇痛作用(P<0.05、0.01),益气养血作用较弱,活血化瘀作用不明显;痹祺胶囊高剂量组具有显著的益气养血、活血化瘀、抗炎及外周镇痛作用,中枢镇痛作用不明显,具体表现在可提高外周血细胞数量、外周血中EPO含量及改善脾脏T淋巴细胞CD4^(+)、CD8^(+)分型(P<0.05、0.01),改善毛细血管流速、降低不同切速下全血黏度,并延长TT(P<0.05、0.01),抑制巴豆油引起的耳肿及醋酸诱发的疼痛反应(P<0.05、0.01)。结论痹祺胶囊具有明显的益气养血、活血化瘀及抗炎镇痛作用,并为明确其功能药味奠定药效基础。Objective To explore the pharmacodynamic effect of Biqi Capsule(痹祺胶囊)on benifiting qi and nourishing blood,promoting blood circulation to dissipate blood stasis,and anti-inflammation and analgesia.Methods The effects of benifiting qi and nourishing blood,promoting blood circulation and removing blood stasis of Biqi Capsule were respectively observed in rat models of qi and blood deficiency and microcirculation disturbance.The anti-inflammatory effect of Biqi Capsule was observed by acute otoedema model in mice.The analgesic effect of Biqi Capsule was observed by hot plate/writhing model in mice.The animals were respectively divided into blank group(except for writhing experiment),model group(except for hot plate experiment),positive drug group,Biqi Capsule low-dose group(equivalent to clinical dosage),Biqi Capsule medium-dose group(equivalent to two times of clinical dosage),Biqi Capsule high-dose group(equivalent to four times of clinical dosage).Samples were collected 1 h after the last administration of Biqi Capsule,the number of peripheral blood cells in the whole blood was detected,the content of erythropoietin(EPO)in serum was detected by ELISA,and spleen CD4^(+)/CD8^(+)ratio was detected by flow cytometry,which were used to preliminatively evaluate the effect of benifiting qi and nourishing blood of Biqi Capsule.The flow velocity of mesenteric capillaries of rats was detected,the viscosity of whole blood was analyzed,thrombin time(TT)kit was used to analyze the coagulation time of serum,so as to preliminically evaluate the effect of Biqi Capsule on promoting blood circulation and removing blood stasis.Results Biqi Capsule low-dose group(equivalent to clinical dose)had significant effects of benifiting qi and nourishing blood,antiinflammatory and analgesic effects(P<0.05,0.01),the effects of promoting blood circulation and removing blood stasis was not obvious.Biqi Capsule medium-dose group(equivalent to two times of clinical dose)showed significant anti-inflammatory and analgesic effects(P<0.05,0.0

关 键 词：痹祺胶囊 气血两虚 微循环障碍 疼痛 炎症 

分 类 号：R285.5[医药卫生—中药学]