The recombinant truncated envelope protein of West Nile virus adjuvanted with Alum/CpG induces potent humoral and T cell immunity in mice  

作　　者：Yongping Du Yao Deng Ying Zhan Ren Yang Jiao Ren Wen Wang Baoying Huang Wenjie Tan 

机构地区：[1]School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou 325035,China [2]National Health Commission(NHC)Key Laboratory of Biosafety,National Institute for Viral Disease Control and Prevention,Beijing 102206,China

出　　处：《Biosafety and Health》2023年第5期300-307,共8页生物安全与健康（英文）

基　　金：the National Key Research and Development Program of China(2022YFC2304100,2022YFC2303401,2016YFD0500301).

摘　　要：West Nile virus(WNV)is a mosquito-transmitted flavivirus distributed globally for decades and can cause disease in humans and animals.So far,no WNV vaccine has been licensed for human use.Therefore,the development of novel candidate vaccines and the improvement of vaccination strategies is imperative.As the WNV envelope(E)glycoprotein plays an important role in mediating viral binding to cellular receptors and virus-cell membrane fusion,it is a critical target for the host humoral response.Here,we prepared a recombinant truncated envelope protein of WNV(rWNV-80E)and developed a WNV subunit vaccine formulation with a combination of aluminum hydroxide(alum)and a synthetic oligonucleotide CpG as adjuvants.C57BL/6 mice were immunized twice intramuscularly at 28-day intervals with 5μg purified rWNV-80E adjuvanted with Alum/CpG.WNV E-specific IgG was detected by enzyme-linked immunosorbent assay and neutralizing antibodies(nAbs)was detected using single-round infectious particles of WNV.Furthermore,T cell immunity was detected by enzyme-linked immunospot assay and intracellular cytokine staining assay.Notably,rWNV-80E was highly immunogenic and elicited potent humoral and cell immunity,as evidenced by significant levels of IFN-γ and TNF-αsecretion in the T cells of mice.In summary,the Alum/CpG-adjuvanted rWNV-80E subunit vaccine elicited potent and balanced B-and T-cell immunity in mice,and therefore it is a promising candidate vaccine that warrants further investigation for use in human or veterinary applications.

关 键 词：West Nile virus Envelope protein Vaccine Alum/CpG adjuvant Immunity 

分 类 号：R392[医药卫生—免疫学]