耐盐芦苇高频遗传转化体系的建立  

Establishment of High Frequency Regeneration System of Phragmites australis

作　　者：王美玲 韩建玮 陈方军 尹海波[1] 张侠[1] WANG Meiing;HAN Jianwei;CHEN Fangjun;YIN Haibo;ZHANG Xia(School of Life Sciences,Yantai University,Yantai 264005,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2024年第1期69-73,共5页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：山东省“渤海粮仓”科技示范工程升级版计划项目(2019BHLC001);国家自然科学基金资助项目(31000128);山东自然科学基金资助项目(ZR2011CQ013)。

摘　　要：以耐盐芦苇(Phragmites australis)种子愈伤组织为材料,以根瘤农杆菌EHA105为媒介建立高效的芦苇遗传转化体系。结果表明:在潮霉素浓度为50 mg/L、6-BA浓度为0.8 mg/L时不定芽分化率达到最高。将沉默载体pFGC1008-GRPi通过根瘤农杆菌EHA105在芦苇愈伤组织中进行遗传转化,共获得8个抗性株系,转化率为3.9%。实时荧光定量PCR检测显示6个株系实现了基因沉默,表明该转化体系高效可用。An efficient genetic transformation system of P.australis is established by using the callus of salt tolerant reed(P.australis)seeds as materials and Agrobacterium tumefaciens EHA105 as vector.The results show that the differentiation rate of adventitious buds reaches the highest with 50 mg/L Hyp and 0.8 mg/L 6-BA.The silencing vector pFGC1008-GRPi is transformed into reed callus by EHA105.A total of 8 positive transformation lines are ob-tained,with a transformation rate of 3.9%.Real time fluorescent quantitative PCR detection shows that 6 strains a-chieved gene silencing,indicating that the transformation system is convenient and available.

关 键 词：芦苇 根瘤农杆菌 遗传转化 

分 类 号：S181[农业科学—农业基础科学]