人参皂苷Rb1对肺动脉高压大鼠钙库操纵性钙内流的影响  

Effect of ginsenoside Rb1 on store-operated calcium entry in rats with pulmonary hypertension

作　　者：黄循铷 郭静一[1,3] 左微 陈亚乐 王瑞幸 何瑞岚[1,4] Huang Xunru;Guo Jingyi;Zuo Wei;Chen Yale;Wang Ruixing;He Ruilan(The Key Laboratory of Fujian Province Universities on Ion Channel and Signal Transduction in Cardiovascular Diseases,School of Basic Medical Sciences,Fujian Medical University,Fuzhou 350122,China;Department of Gastroenterology,the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China;Department of Pathology,Henan Infectious Diseases Hospital,Zhengzhou 450015,China;Department of Physiology and Pathophysiology,School of Basic Medical Sciences,Fujian Medical University,Fuzhou 350122,China)

机构地区：[1]福建医科大学基础医学院心血管病离子通道信号调控福建省高校重点实验室,福州350122 [2]福建医科大学附属第一医院消化内科,福州350005 [3]河南省传染病医院病理科,郑州450015 [4]福建医科大学基础医学院生理学与病理生理学系,福州350122

出　　处：《广西医科大学学报》2023年第12期2004-2009,共6页Journal of Guangxi Medical University

基　　金：福建省自然科学基金资助项目(No.2023J01547,No.2020J01616)。

摘　　要：目的:观察在体人参皂苷Rb1预处理对慢性低氧(CH)肺动脉高压大鼠肺动脉平滑肌细胞钙库操纵性钙内流(SOCE)的影响。方法:将30只雄性SD大鼠随机分为正常对照组(control组)、CH肺动脉高压模型组(CH组)和Rb1(30 mg/kg)组,每组10只。检测各组右心室收缩压、右心室质量指数和肺动脉血管环张力。体外培养大鼠肺动脉平滑肌细胞,采用细胞动态荧光检测游离Ca^(2+)浓度。分别采用实时荧光定量PCR(RT-qPCR)和western blotting检测基质相互作用分子2(STIM2)和钙释放激活钙调节因子2(Orai2)基因和蛋白表达。结果:与CH组比较,Rb1组大鼠右心室收缩压和右心室质量指数均明显降低(P<0.01),环匹阿尼酸(CPA)诱发的肺动脉收缩张力和钙内流量明显减少(P<0.01),STIM2和Orai2 mRNA和蛋白相对表达量明显降低(P<0.05)。结论:Rb1可明显改善CH肺动脉高压大鼠的肺动脉压,其作用机制可能与减弱肺动脉SOCE功能和STIM2和Orai2表达有关。Objective:To observe the effect of pretreatment with ginsenoside Rb1 in vivo on store-operated calci-um entry(SOCE)in pulmonary artery smooth muscle cells of rats with chronic hypoxia(CH)pulmonary hyper-tension.Methods:Thirty male SD rats were randomly divided into normal control group(control group),CH pul-monary hypertension model group(CH group)and Rb1(30 mg/kg)group,with 10 rats in each group.The right ventricular systolic pressure,right ventricular mass index,and pulmonary artery vascular tension in each group were measured.The pulmonary artery smooth muscle cells of rats were cultured in vitro,and the free Ca^(2+) concen-tration was detected by cell dynamic fluorescence.The gene and protein expressions of stromal interaction mole-cule 2(STIM2)and calcium release-activated calcium modulator 2(Orai2)were detected by real-time quantita-tive PCR(RT-qPCR)and western blotting.Results:Compared with the NC group,the right ventricular systolic pressure and right ventricular mass index of the rats in the Rb1 group were significantly lower(P<0.01),the cyclopiazonic acid(CPA)-induced pulmonary ar-tery constriction tension and intracellular calcium in-flux were significantly reduced(P<0.01),and STIM2,Orai2 mRNA as well as protein expression of relative amount were significantly decreased(P<0.05).Conclusion:Rb1 can significantly improve the pulmonary artery pressure in CH rats with pulmonary hyperten-sion,and the mechanism may be related to the reduction of SOCE function and the expression of STIM2 and Orai2 in pulmonary artery.

关 键 词：肺动脉高压 慢性低氧 人参皂苷RB1 钙库操作性钙内流 肺动脉 

分 类 号：R965.2[医药卫生—药理学]