丹参葛根提取物对阿霉素引发小鼠心力衰竭的作用  

EFFECT OF SALVIA MILTIORRHIZA AND RADIX PUERARIAE EXTRACT ON DOXORUBICIN-INDUCED HEART FAILURE IN MICE

作　　者：张梦瑶 张咪 韩彦弢[1] 赵文文[1] ZHANG Mengyao;ZHANG Mi;HAN Yantao;ZHAO Wenwen(School of Basic Medicine,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院,山东青岛266071

出　　处：《青岛大学学报（医学版）》2023年第6期894-897,共4页Journal of Qingdao University(Medical Sciences)

基　　金：山东省中医药科技项目(2021M137)。

摘　　要：目的探究丹参葛根提取物(DG)对阿霉素(DOX)引发的心力衰竭小鼠(DOX-HF小鼠)的心脏保护作用。方法将32只6周龄雄性C57BL/6J小鼠随机分为对照组(A组)、模型组(B组)、低剂量给药组(C组)和高剂量给药组(D组),每组8只。B组、C组和D组腹腔注射DOX 4周(每周5 mg/kg),C组和D组在建模开始时每日1次灌胃DG水溶液,灌胃剂量分别为50 mg/kg和100 mg/kg;A组、B组每日灌胃相同剂量的生理盐水。采用超声心动图检测评估心脏机械功能改变情况,苏木精-伊红(HE)染色和Masson染色观察心肌组织病理学变化,酶联免疫吸附法(ELISA)检测血清中氨基末端脑钠肽前体(NT-proBNP)、肌钙蛋白I(cTnI)、肿瘤坏死因子(TNF-α)和白细胞介素-6(IL-6)水平。结果超声心动图检测结果显示,与A组相比,B组左室射血分数(EF)、左室短轴缩短率(FS)显著下降,左室舒张末期容积(LVEDV)和左室收缩末期容积(LVESV)显著升高;与B组相比,C组和D组EF和FS显著升高,LVEDV和LVESV显著下降,差异均有显著性(F=7.66~29.30,P<0.05)。HE染色结果显示,与A组相比,B组心肌组织结构明显异常,心肌细胞排列紊乱且水肿,空泡化严重;C组和D组心肌细胞排列轻度紊乱,可见少许空泡。Masson染色结果显示,与A组相比,B组胶原沉积显著增加,C组和D组胶原沉积较B组明显减少。ELISA检测显示,与A组比较,B组NT-proBNP、cTnI、TNF-α和IL-6水平显著升高,C组和D组NT-proBNP、cTnI、TNF-α和IL-6水平较B组显著下降,差异有显著性(F=17.54~32.59,P<0.05)。结论DG能够有效保护DOX-HF小鼠的心脏功能。Objective To explore the cardioprotective effect of Salvia miltiorrhiza-Radix puerariae extract(DG)on mice with heart failure induced by doxorubicin(DOX-HF mice).Methods In the experiment,32 C57BL/6J mice(6 weeks old,male)were randomly divided into four groups:control group(group A),model group(group B),low-dose DG group(group C),and high-dose DG group(group D)(n=8 per group).Mice form groups B,C,and D received an intraperitoneal injection of DOX for 4 weeks(5 mg/kg per week).Meanwhile,mice in groups C and D were intragastrically given DG water solution once daily at the beginning of modeling with doses of 50 and 100 mg/kg,respectively.Mice in groups A and B were intragastrically given the same dose of normal saline daily.The changes in cardiac mechanical function were evaluated by echocardiography;myocardial histopathological changes were observed by hematoxylin-eosin(HE)staining and Masson staining;enzyme-linked immunosorbent assay(ELISA)was used to measure levels of serum N-terminal pro-brain natriuretic peptide(NT-proBNP),cardiac troponin I(cTnI),tumor necrosis factor-α(TNF-α),and interleukin-6(IL-6).Results Echocardiography data showed that compared with group A,group B had significantly decreased left ventricular ejection fraction and left ventricular fractional shortening and significantly increased left ventricular end-diastolic volume and left ventricular end-systolic volume.Above phenomena that occurred in group B were significantly reversed in groups C and D(F=7.66-29.30,P<0.05).Furthermore,the data from HE staining showed that significantly abnormal myocardial tissue structure characterized by disordered and edematous myocardial cells especially with se-rious vacuolation was observed in group B compared with group A.Meanwhile,above morphological changes were alle-viated with DG treatment in mice from groups C and D.Besides,the data from Masson staining showed that collagen deposition was significantly increased in group B compared with group A but was significantly reduced in groups C and D compared

关 键 词：多柔比星 心力衰竭 丹参葛根提取物 炎症 

分 类 号：R969[医药卫生—药理学]