Comparative study of type 2 diabetes mellitus-associated gut microbiota between the Dai and Han populations  

作　　者：Ling-Tong Tang Lei Feng Hui-Ying Cao Rui Shi Bei-Bei Luo Yan-Bi Zhang Yan-Mei Liu Jian Zhang Shuang-Yue Li 

机构地区：[1]Department of Clinical Laboratory,Yan’an Hospital Affiliated to Kunming Medical University,Kunming 650051,Yunnan Province,China [2]Department of Clinical Laboratory,Sixth Affiliated Hospital of Kunming Medical University,Kunming 650051,Yunnan Province,China

出　　处：《World Journal of Diabetes》2023年第12期1766-1783,共18页世界糖尿病杂志（英文版）（电子版）

基　　金：the National Natural Science Foundation of China,No.82160402;Special Fund for Training Leading Medical Talents in Yunnan Province,China,No.L-2019022。

摘　　要：BACKGROUND The global prevalence of type 2 diabetes mellitus(T2DM)is increasing.T2DM is associated with alterations of the gut microbiota,which can be affected by age,illness,and genetics.Previous studies revealed that there are discriminating microbiota compositions between the Dai and the Han populations.However,the specific gut microbiota differences between the two populations have not been elucidated.AIM To compare the gut microbiota differences in subjects with and without T2DM in the Dai and Han populations.METHODS A total of 35 subjects of the Han population(including 15 healthy children,8 adult healthy controls,and 12 adult T2DM patients)and 32 subjects of the Dai population(including 10 healthy children,10 adult healthy controls,and 12 adult T2DM patients)were enrolled in this study.Fasting venous blood samples were collected from all the subjects for biochemical analysis.Fecal samples were collected from all the subjects for DNA extraction and 16S rRNA sequencing,which was followed by analyses of the gut microbiota composition.RESULTS No significant difference in alpha diversity was observed between healthy children and adults.The diversity of gut microbiota was decreased in T2DM patients compared to the healthy adults in both the Dai and Han populations. There was a significant difference in gut microbiota between healthy children and healthy adults in the Hanpopulation with an increased abundance of Bacteroidetes and decreased Firmicutes in children. However, thisdifference was less in the Dai population. Significant increases in Bacteroidetes in the Han population and Proteobacteriain the Dai population and decreases in Firmicutes in both the Han and Dai population were observed inT2DM patients compared to healthy adults. Linear discriminant analysis Effect Size analysis also showed that thegut microbiota was different between the Han and Dai populations in heathy children, adults, and T2DM patients.Four bacteria were consistently increased and two consistently decreased in the Han population compar

关 键 词：Gut microbiota Type 2 diabetes mellitus Dai population Han population GENETICS ETHNIC 

分 类 号：R587.1[医药卫生—内分泌]