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作 者:梁书卿 周晓慧[2] 杨艳 张静娴 刘松瑜 刘军[2] 庄勇 LIANG Shuqing;ZHOU Xiaohui;YANG Yan;ZHANG Jingxian;LIU Songyu;LIU Jun;ZHUANG Yong(College of Horticulture,Nanjing Agricultural University,Nanjing 210095;Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014)
机构地区:[1]南京农业大学园艺学院,南京210095 [2]江苏省农业科学院蔬菜研究所/江苏省高效园艺作物遗传改良重点实验室,南京210014
出 处:《植物遗传资源学报》2024年第1期120-128,共9页Journal of Plant Genetic Resources
基 金:江苏省自然科学基金(BK20221429);江苏省重点研发计划项目(BE2021377);江苏省农业科技自主创新项目(CX(22)3160)。
摘 要:Whirly是一类能与单链DNA分子结合的植物特异转录因子,在细胞核以及细胞器内都起着广泛且复杂的作用。为探究茄子Whirly基因的功能和进化关系,本研究开展了基因家族成员生物信息学鉴定,包括系统进化关系、基因结构、保守基序及启动子中的顺式作用元件,分析了其在不同组织、外源激素处理和逆境胁迫下表达模式。结果表明,茄子Whirly基因家族包含两个家族成员,分别命名为SmWHY1和SmWHY2,它们与番茄中Whirly基因亲缘关系最近。SmWHY1和SmWHY2在不同组织中均能表达,在叶片中SmWHY1的表达水平高于SmWHY2,在其他组织中SmWHY2的表达水平高于SmWHY1。两个基因的表达均受到脱落酸、茉莉酸甲酯、水杨酸、低温胁迫和病原菌诱导,但不同处理或基因型响应程度存在差异,在抗绵疫病种质G114中能够保持较高的表达水平。以上结果表明SmWHY1和SmWHY2可能在调控茄子生长发育和逆境响应中具有重要作用。Whirly is a class of plant specific transcription factors that can bind to single-stranded DNA molecules and play a wide and complex role in both nucleus and organelles.In order to explore the functional and evolutionary relationships of Whirly genes in eggplant,we applied the bioinformatics approach to identify Whirly genes,including the phylogenetic relationships,gene structure,conservative motifs and cis-acting elements in promoters.Their transcriptional profiles were also analyzed in different tissues,or under treatments of exogenous hormone,low temperature and pathogen infection.The results showed that the Whirly gene family of eggplant contained two members,named SmWHY1 and SmWHY2,which were closely related to the Whirly genes in tomato.Both SmWHY1 and SmWHY2 expressed in different tissues,and the transcripts of SmWHY1 in leaves were higher than that of SmWHY2 in other tissues.The induction at two genes was detected under treatments(abscisic acid,methyl jasmonate,salicylic acid,low temperture and pathogen),whereas the responses were variable in gene and/or by treatments.In G114,a Phytophthora capsici resistant germplasm,two genes showed relatively high expression.Collectively,these results indicated that SmWHY1 and SmWHY2 may play an important role in the regulation of growth,development,and response to stress.
关 键 词:茄子 Whirly转录因子 生物信息学 表达分析
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