通过骨髓移植建立骨髓源性细胞特异性基因修饰小鼠模型  

Establishment of a bone marrow-derived cell-specific gene-modified mouse model by bone marrow transplantation

作　　者：余钢 陈锡坚 郭锦伟 葛佳辉 周应毕 刘斌 YU Gang;CHEN Xijian;GUO Jinwei;GE Jiahui;ZHOU Yingbi;LIU Bin(Cardiovascular Center,Shantou University Medical College,Shantou 515041,China)

机构地区：[1]汕头大学医学院心血管中心,广东汕头515041

出　　处：《汕头大学医学院学报》2023年第4期193-196,204,共5页Journal of Shantou University Medical College

基　　金：国家自然科学基金(82070506)。

摘　　要：目的:通过骨髓移植建立骨髓源性细胞特异性基因修饰小鼠模型。方法:将具有相同遗传背景的供体小鼠(C57BL/6野生型小鼠)的骨髓细胞移植到经X射线辐照的受体小鼠(C57BL/6 Ep3^(-/-)小鼠)中,通过不同辐射剂量和方式的对比,确定基因修饰小鼠骨髓移植实验的最适辐照方案。提取受体小鼠血液和鼠尾组织DNA进行基因型鉴定和嵌合率分析。结果:受体小鼠经合适剂量的X射线辐照(辐射剂量为8.0 Gy,分2次照射,每次4.0 Gy,中间间隔6 h),小鼠存活率可达到(74.3±2.4)%,嵌合率(95.77±0.52)%,符合骨髓移植实验要求。结论:成功建立了符合骨髓移植实验要求的骨髓源性细胞特异性基因修饰小鼠模型,可用于心血管及炎症等相关的研究工作。Objective:To establish a mouse model of bone marrow-derived cell-specific gene modification by bone marrow transplantation.Methods:Bone marrow cells from donor mice(C57BL/6 wild-type)with the same genetic background were transplanted into X-ray irradiated recipient mice(C57BL/6 Ep3^(-/-)mice),and the optimal irradiation regimen for the bone marrow transplantation experiments in genetically modified mice was determined by the comparison of different radiation dosages and modalities.Blood and tail tissue DNA of recipient mice were extracted for genotype identification and chimerism rate analysis.Results:After recipient mice were irradiated by X-rays with appropriate dose(radiation dose of 8.0 Gy,irradiated in 2 times,4.0 Gy each time,with an interval of 6 h),the survival rate of the mice could reach(74.3±2.4)%and the chimerism rate(95.77±0.52)%,which was in accordance with the requirements of bone marrow transplantation experiments.Conclusion:A bone marrow-derived cell-specific gene-modified mouse model that meets the requirements of bone marrow transplantation experiments has been successfully established,which can be used for cardiovascular and inflammation-related research work.

关 键 词：骨髓移植 骨髓源性细胞 辐射 嵌合率 基因修饰小鼠 

分 类 号：R457.7[医药卫生—治疗学]