振源胶囊对慢性心力衰竭患者循环微粒的影响  

Effect of Zhenyuan capsule on circulating microparticles in patients with chronic heart failure

作　　者：李文波[1] 常凤军[1] 王亚萍[1] 杨禹娟[1] 王群让 尤红俊 LI Wenbo;CHANG Fengjun;WANG Yaping;YANG Yujuan;WANG Qunrang;YOU Hongjun(Department of Cardiology,Shaanxi Provincial People’s Hospital,Xi’an 710068;Department of Cardiology,Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang Shaanxi 712000,China)

机构地区：[1]陕西省人民医院心血管内科,西安710068 [2]陕西中医药大学附属医院心血管内科,陕西咸阳712000

出　　处：《临床与病理杂志》2023年第10期1809-1815,共7页Journal of Clinical and Pathological Research

基　　金：陕西省自然科学基金(2017JQ8025);陕西省分子心脏学重点实验室基金(KLMC-2018-05)。

摘　　要：目的:循环微粒(microparticles,MPs)在心血管系统中发挥重要作用,而振源胶囊在治疗心力衰竭方面有独到优势。本研究探讨慢性心力衰竭患者服用振源胶囊前后体内MPs含量及功能的变化。方法:选取2018年6月至2019年3月就诊于陕西中医药大学附属医院心血管内科的32例慢性心力衰竭患者为研究对象。抽取患者服用振源胶囊前及服用振源胶囊12周后的空腹静脉血,离心提取患者体内的MPs并检测其浓度及来源,而后检测服药前后MPs对离体SD大鼠胸主动脉血管舒张功能的影响和内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)、一氧化氮(nitric oxide,NO)表达的影响。结果:与服药前比较,服用振源胶囊12周后患者体内MPs水平变化差异无统计学意义(P>0.05)。振源胶囊在一定程度上减轻了MPs对离体大鼠血管舒张功能的损害,同时还部分抑制了MPs对离体大鼠胸主动脉中eNOS和NO的下调作用(均P<0.05)。结论:振源胶囊可能部分通过抑制MPs对内皮功能的损害发挥其在慢性心力衰竭中的心血管保护功效。Objective:Circulating microparticles(MPs)play an important role in the cardiovascular system,while Zhenyuan capsules have unique advantages in the treatment of chronic heart failure(CHF).This study explores the changes in the content and function of MPs in CHF patients before and after taking Zhenyuan capsule.Methods:A total of 32 patients with CHF,who visited the Department of Cardiology at the Affiliated Hospital of Shaanxi University of Chinese Medicine from June 2018 to March 2019,were selected as research subjects.Fasting venous blood samples were collected from patients before and after 12 weeks of Zhenyuan capsule administration.MPs were extracted from the patient’s blood,and their concentration and source were determined.Subsequently,the effects of MPs on the vasodilation function of isolated SD rat thoracic aorta and the expression of endothelial nitric oxide synthase(eNOS)and nitric oxide(NO)production were detected.Results:There was no statistically significant difference in the level of MPs in patients’blood before and after 12 weeks of Zhenyuan capsule administration(P>0.05).Zhenyuan capsules partially alleviated the impairment of vascular dilation function induced by MPs in isolated rat aortas.Additionally,Zhenyuan capsules partially inhibited the downregulation of eNOS and NO production in the isolated rat thoracic aorta induced by MPs(both P<0.05).Conclusion:Zhenyuan capsules may exert cardiovascular protective effects in CHF,partially by inhibiting the damage to endothelial function caused by MPs.

关 键 词：慢性心力衰竭 循环微粒 一氧化氮 内皮功能 

分 类 号：R541.6[医药卫生—心血管疾病]