关中奶山羊发情期不同时段宫颈阴道粘液非靶向代谢组学比较分析研究  

Untargeted Metabolomics Comparative Analysis of Cervical and Vaginal Mucus in Different Estrus Periods in Guanzhong Dairy Goats

作　　者：冉本康 李宇 贤明 郭松茂 王立强[1] 孟智利 李延华 王军宁 李芳娥 杜烨青 胡建宏[1] RAN Benkang;LI Yu;XIAN Ming;GUO Songmao;WANG Liqiang;MENG Zhili;LI Yanhua;WANG Junning;LI Fange;DU Yeqing;HU Jianhong(College of Animal Science and Technology,Northwest A&F University,Yangling Shaanxi 712100,China;Fuping Huatuo Animal Husbandry Technology Co.LTD,Fuping Shaanxi 711700,China;Shaanxi Guanzhong Dairy Goat Professional Cooperative,Fuping Shaanxi 711700,China;Shaanxi Aonik Dairy Goat Breeding Co.LTD,Fuping Shaanxi 711700,China)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]富平县华拓畜牧科技有限公司,陕西富平711700 [3]陕西关中奶山羊专业合作社,陕西富平711700 [4]陕西澳尼克奶山羊育种有限公司,陕西富平711700

出　　处：《家畜生态学报》2024年第2期39-46,共8页Journal of Domestic Animal Ecology

基　　金：陕西省重点研发计划项目(2020ZDLNY02-04);陕西省农业专项资金项目(畜牧专项资金-XN10)。

摘　　要：为了揭示关中奶山羊自然发情状态下发情期生殖道代谢特征,探索关中奶山羊发情期特异性化学信号,为精准发情鉴定和适时人工授精等繁殖工作提供代谢机制层面的理论依据,以3只2~3岁经产母羊为研究对象,采集关中奶山羊发情期0~12 h(A组),12~24 h(B组),24~36 h(C组)的宫颈阴道粘液,采用气相色谱-质谱法(GC-MS)测定关中奶山羊发情期宫颈阴道粘液中的代谢产物。结果显示,来自发情期不同时段的关中奶山羊宫颈阴道粘液中共检出221种不同的代谢产物,其主要分类是碳水化合物、脂质、氨基酸、核苷酸、异生素、辅助因子和维生素、肽类、能量等;0~12 h VS 12~24 h的差异代谢物有6种,12~24 h VS 24~36 h的差异代谢物有3种,0~12 h VS 24~36 h的差异代谢物有14种;这些差异代谢物主要富集在半乳糖代谢、碳水化合物消化吸收、矿物质吸收、甘油酯代谢、ABC蛋白转运、嘧啶代谢等通路上。In order to reveal the metabolic characteristics of genital tract in natural estrus Guanzhong dairy goats,explore the specific chemical signals in estrus Guanzhong dairy goats,and provide a theoretical basis in terms of metabolic mechanism for accurate identification of estrus and timely artificial insemination,this study selected three 2-to 3-year-old Guanzhong dairy ewes to collect their cervical and vaginal mucus from 0-12 h,12-24 h and 24-36 h during estrus.Meteorological chromatography-mass spectrometry(GC-MS)was used to determine the metabolites in the cervical and vaginal mucus of Guanzhong dairy goats during estrus.The results showed that 221 different metabolites were detected in the cervical and vaginal mucus of Guanzhong dairy goats from different periods of estrus,and they were mainly carbohydrate,lipid,amino acid,nucleotide,xenobiotics,cofactors and vitamins,peptide and energy,etc.There were 6 different metabolites in 0-12 h VS 12-24 h,3 different metabolites in 12-24 h VS 24-36 h,and 14 different metabolites in 0-12 h VS 24-36 h.These differential metabolites were mainly enriched in pathways of galactose metabolism,carbohydrate digestion and absorption,mineral absorption,glycerolipid metabolism,ABC transporters,and pyrimidine metabolism.

关 键 词：关中奶山羊 自然发情 发情周期 宫颈阴道粘液 差异代谢物 

分 类 号：S827.34[农业科学—畜牧学]